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4,5-DIAMINOFLUORESCEIN DIACETATE, also known as DAF-2 DA, is a cell-permeable fluorescent probe used for the detection of nitric oxide (NO) in various applications. It is a diacetate derivative of the fluorescent molecule fluorescein, which allows it to easily penetrate cell membranes and be deacetylated by cellular esterases to form the active diaminofluorescein (DAF-2). This active form can then react with nitric oxide, resulting in a fluorescent product that can be detected and quantified.

205391-02-2

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205391-02-2 Usage

Uses

Used in Biomedical Research:
4,5-DIAMINOFLUORESCEIN DIACETATE is used as a highly sensitive probe for the real-time detection of nitric oxide (NO) in vivo and in cell culture experiments. Its cell permeability allows for the monitoring of NO production in living cells, making it a valuable tool for studying the role of NO in various physiological and pathological processes.
Used in Fluorimetric Detection:
In the field of analytical chemistry, 4,5-DIAMINOFLUORESCEIN DIACETATE is used as a fluorimetric detection agent for nitric oxide. The reaction between DAF-2 DA and NO produces a fluorescent product, which can be measured using fluorescence spectroscopy. This method provides a sensitive and specific way to quantify NO levels in various samples, including biological tissues, cell lysates, and culture media.
Used in Pharmaceutical and Environmental Research:
4,5-DIAMINOFLUORESCEIN DIACETATE can also be employed in pharmaceutical research for the development of drugs targeting nitric oxide pathways, as well as in environmental studies to monitor NO levels in air, water, and soil samples. Its versatility and sensitivity make it a valuable tool for a wide range of applications in both research and industry.

Check Digit Verification of cas no

The CAS Registry Mumber 205391-02-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,0,5,3,9 and 1 respectively; the second part has 2 digits, 0 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 205391-02:
(8*2)+(7*0)+(6*5)+(5*3)+(4*9)+(3*1)+(2*0)+(1*2)=102
102 % 10 = 2
So 205391-02-2 is a valid CAS Registry Number.

205391-02-2SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 12, 2017

Revision Date: Aug 12, 2017

1.Identification

1.1 GHS Product identifier

Product name 4,5-Diaminofluorescein diacetate

1.2 Other means of identification

Product number -
Other names (6'-acetyloxy-5,6-diamino-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:205391-02-2 SDS

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