206443-06-3

Basic information

• Product Name: 4-Methylumbelliferylb-D-galactopyranoside-6-sulphatesodiumsalt
• Synonyms: 4-Methylumbelliferylb-D-galactopyranoside-6-sulphatesodiumsalt;4-Methylumbelliferyl β-D-galactopyranoside-6-sulphate sodium salt;4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate Sodium Salt;4-Methyl-7-[(6-O-sulfo-β-D-galactopyranosyl)oxy]-;4-Methyl-7-[(6-O-sulfo-beta-D-galactopyranosyl)oxy]-2H-1-benzopyran-2-one monosodium salt;[(3R,4S,5S,6S)-3,4,5-trihydroxy-6-(4-methyl-2-oxo-chromen-7-yl)oxy-tetrahydropyran-2-yl]methoxysulfonyloxysodium
• CAS NO: 206443-06-3
• Molecular Formula: C16H17NaO11S
• Molecular Weight: 440.35435
• EINECS: 1533716-785-6
• Product Categories: Carbohydrates & Derivatives;Fluorescent Labels & Indicators
• Mol File: 206443-06-3.mol
• Article Data: 0

Chemical Properties

• Melting Point: 196-198?C
• Appearance: /
• Storage Temp.: -20°C Freezer, Under Inert Atmosphere
• Solubility: Methanol (Slightly, Sonicated), Water (Slightly)
• Stability: -20?C Freezer, Under Inert Atmosphere
• CAS DataBase Reference: 4-Methylumbelliferylb-D-galactopyranoside-6-sulphatesodiumsalt(CAS DataBase Reference)
• NIST Chemistry Reference: 4-Methylumbelliferylb-D-galactopyranoside-6-sulphatesodiumsalt(206443-06-3)
• EPA Substance Registry System: 4-Methylumbelliferylb-D-galactopyranoside-6-sulphatesodiumsalt(206443-06-3)

Safety Data

• Hazardous Substances Data: 206443-06-3(Hazardous Substances Data)

Usage

Description

4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) (4-MU-Gal-6S) is a fluorogenic substrate used to quantify N-acetylgalactosamine-6-sulphatase (GALNS) activity. It is a white solid that exhibits pH-dependent fluorescence, with excitation maxima of 320 and 360 nm at low and high pH, respectively, and an emission maximum ranging from 445 to 455 nm.

Uses

Used in Enzyme Activity Assays:
4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) is used as a substrate for the determination of galactose-6-sulfate sulfatase activity. It is cleaved by the enzyme to release the fluorescent moiety 4-MU, allowing for the quantification of enzyme activity.
Used in Lysosomal Storage Disorder Diagnosis:
4-MU-Gal-6S is used in the diagnosis of Mucopolysaccharidosis IV A (Morquio disease type A), a lysosomal storage disorder characterized by a deficiency of GALNS enzyme activity. The substrate can be used to assess GALNS activity in a very small blood volume, helping to determine the extent of deficiency.
Used in Research and Development:
4-MU-Gal-6S is utilized in research and development for the study of lysosomal storage disorders, enzyme kinetics, and the development of potential therapeutic agents targeting GALNS deficiency. Its fluorescence properties make it a valuable tool for monitoring enzyme activity and screening for potential enzyme modulators.

InChI:InChI=1/C16H18O11S.Na/c1-7-4-12(17)26-10-5-8(2-3-9(7)10)25-16-15(20)14(19)13(18)11(27-16)6-24-28(21,22)23;/h2-5,11,13-16,18-20H,6H2,1H3,(H,21,22,23);/q;+1/p-1/t11?,13-,14-,15-,16+;/m0./s1/rC16H17NaO11S/c1-7-4-12(18)26-10-5-8(2-3-9(7)10)25-16-15(21)14(20)13(19)11(27-16)6-24-29(22,23)28-17/h2-5,11,13-16,19-21H,6H2,1H3/t11?,13-,14-,15-,16+/m0/s1