21199-28-0 Usage
Physical Appearance
Yellow tetrazolium dye
Application
Used in the MTT assay for determining cell viability and proliferation in biological research
Assay Principle
Colorimetric assay measuring the activity of enzymes that reduce MTT to formazan, a purple compound
Cellular Uptake
Readily taken up by living cells
Metabolic Reduction
Reduced by mitochondrial dehydrogenases within living cells
Formazan Formation
Formed as a result of reduction, which is solubilized
Measurement
Absorbance of formazan measured to determine cell viability
Cytotoxic Effects
Shown to have cytotoxic effects in some cell types
Caution in Use
Caution advised due to potential cytotoxicity
Metabolic Dependency
Reduction dependent on cellular metabolism
Experimental Variability
Potential variability in results due to influence of experimental conditions on cellular metabolism
Check Digit Verification of cas no
The CAS Registry Mumber 21199-28-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,1,1,9 and 9 respectively; the second part has 2 digits, 2 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 21199-28:
(7*2)+(6*1)+(5*1)+(4*9)+(3*9)+(2*2)+(1*8)=100
100 % 10 = 0
So 21199-28-0 is a valid CAS Registry Number.
