219136-97-7Relevant academic research and scientific papers
The C-terminus shortened analogs of the insect peptide oostatic hormone with accelerated acitivity
Hlavacek, Jan,Tykva, Richard,Bennettova, Blanka,Barth, Tomislav
, p. 131 - 140 (2007/10/03)
Shortened analogs 1a-1f of the oostatic hormone 1g with a decreased number or an omission of the C-terminal Pro residues were synthesized for investigation of their effect on oogenesis in the flesh fly Sarcophaga bullata Parker (Diptera). Peptides 1b-1g were prepared by solid-phase synthesis on polystyrene/divinylbenzene polymer with a 2-chlorotrityl linker using Fmoc/tBu strategy in dimethylformamide (DMF). Tetrapeptide 1a, devoid of all the carboxy-terminal Pro residues, was prepared by stepwise procedure in DMF solution from HCl-H-Ala-OMe using Boc-Pro-OH, Z-Asp(OtBu)-OSu, and Boc-Tyr-OSu. In general, the peptides assayed affected processes of egg development in 20-80% of ovarioles causing changes in the follicular epithelium, proliferation of its nuclei, and cell division toward the inner part of the egg chamber. This process leads to the occurrence of a multilayered follicular epithelium which later fills up almost the whole egg chamber and then degenerates. Such ovarioles are never able to produce eggs. The shortening of the peptide sequence accelerated the observed effects on egg development. The application of the 125I-labeled peptides made possible to express quantitatively the time dependence of the radioactivity distribution in the insect body, including ovaries through which it is transferred to the next F1 generation. The radioactivity was measured in larvaes and, consequently, in newborn flies. For different peptide sequences applied, different radioactivity distributions in the insect body were found using the same radiolabeling.
