219568-89-5Relevant academic research and scientific papers
Synthesis of partially-deuterated 2'-deoxyribonucleoside blocks and their incorporation into an oligo-DNA for simplification of overcrowding and selective enhancement of resolution and sensitivity in the 1H-NMR spectra
Foeldesi, Andras,Maltseva, Tatiana V.,Dinya, Zoltan,Chattopadhyaya, Jyoti
, p. 14487 - 14514 (2007/10/03)
The chemical synthesis of appropriately protected partially-deuterated 2'(R/S),3',5'(R/S)-2H3-2'-deoxyribonucleoside blocks [~43 atoms % 2H at C5'(R), ~57 atom % 2H at C5'(S); ~15 atom % 2H at C2'(R), ~85 atom % 2H at C2'(S) and >99 atom % 2H at C3'] is reported. The availability of these deuterium labelled blocks on large scale has enabled the chemical assemblage of the deuterio isotopomeric 12mer [d(C1G2C3G4A5A6T7T8C9G10C11G12)]2 DNA duplex by standard solid-phase synthesis protocol in order to demonstrate the usefulness of the new 'NMR-window III' approach.
