219648-34-7

Upstream product

• 171284-48-3 2',3',5'-tris-O-acetyl-2-fluoro-O⁶-<2-(4-nitrophenyl)ethyl>inosine 
• 171284-47-2 2′,3′,5′-tri-O-acetyl-O⁶-[2-(4-nitrophenyl)ethyl]guanosine 
• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 219648-31-4 N²-methyl-O⁶-[2-(4-nitrophenyl)ethyl]guanosine 

Relevant academic research and scientific papers

The Synthesis of 2′-O-[(Triisopropylsilyl)oxy] methyl (TOM) Phosphoramidites of Methylated Ribonucleosides (m1G, m2G, m22G, m1I, m3U, m4C, m6A, m62A) for Use in Automated RNA Solid-Phase Synthesis

The straightforward synthesis of eight methylated ribonucleoside phosphoramidites is described. These building blocks allow for incorporation of the naturally occuring nucleosides 1-methylguanosine (m1G), N 2-methylguanosine (m2G), N2N 2-dimethylguanosine (m22G), 1-methylinosine (m1I), 3-methyluridine (m3U), N4- methylcytidine (m4C), N6-methyladenosine (m6A), and N6,N6-dimethyladenosine (m62A) into oligoribonucleotides by automated RNA solid-phase synthesis. In all cases, the ribose 2′-hydroxyl group of the building blocks is masked by the recently introduced [(triisopropylsilyl)oxy]methyl (TOM) group.

The synthesis of RNA containing the modified nucleotides N2- methylguanosine and N6,N6-dimethyladenosine

The phosphoramidites of the naturally occurring modified nucleotides N2-methylguanosine and N6, N6-dimethyladenosine were synthesized and incorporated into short oligoribonucleotides. Described are the syntheses of the phosphoramidites and the procedures used to deprotect oligoribonucleotides in which the O6 of m2G is protected with a 2-(p- nitrophenyl)ethyl group.