22243-97-6Relevant academic research and scientific papers
A simple method of preparing diverse neoagaro-oligosaccharides with β-agarase
Li, Jingbao,Han, Feng,Lu, Xinzhi,Fu, Xiaoyan,Ma, Cuiping,Chu, Yan,Yu, Wengong
, p. 1030 - 1033 (2007)
In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant β-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE. The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and 13C NMR spectroscopy.
13C-N.M.R.-SPECTROSCOPIC INVESTIGATION OF AGAROSE OLIGOMRS
Rochas, Cyrille,Lahaye, Marc,Yaphe, Wilfred,Viet, Minh Tan Phan
, p. 199 - 208 (2007/10/02)
A complete, unambiguous assignment of all of the 13C-n.m.r.-spectral signals of agarose oligomers produced by enzymic hydrolysis has been achieved.The 1J 13C-H coupling constants are reported, and the chemical shifts and coupling constants of both the agarose polymer and oligomers are compared.
