22633-33-6Relevant articles and documents
Nifursol hapten, artificial antigen, and preparation method and application thereof
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Paragraph 0058-0060; 0062-0063, (2021/08/07)
The invention relates to the field of biochemical engineering, and specifically relates to a nifursol hapten, an artificial antigen, and a preparation method and application thereof. The structure of the nifursol hapten is shown as a formula (I), the artificial antigen is obtained after the nifursol hapten is coupled with carrier protein, the artificial antigen is used as an immunogen to prepare a corresponding hybridoma cell 3H10, and a monoclonal antibody secreted by the hybridoma cell 3H10 can be used for detecting nifursol. A monoclonal antibody prepared by immunizing a mouse with an artificial antigen prepared from the nifursol hapten provided by the invention has relatively high sensitivity in detection of nifursol and derivatives thereof, and a new material basis is provided for establishing a rapid, simple, cheap, sensitive and specific nifursol detection method.
Intramolecular hydrogen bonding of o-hydroxyesters and related compounds evaluated by deuterium isotope effects on 13C chemical shifts and principal component analysis
Hansen, Poul Erik,Kamounah, Fadhil S.,Ullah, Saif
, p. 300 - 307 (2008/03/13)
o-Hydroxyaromatic esters, β-hydroxyketoesters, Meldrums acids, other o-hydroxyesters and a few o-hydroxyacid anhydrides are investigated by deuterium isotope effects on 13C chemical shifts, xΔC(OD). Especially the ester carbons show unusual deuterium isotope effects. A principal component analysis based on bond lengths and distances around the hydrogen bond six-membered ring make it possible to predict isotope effects. The ratio nΔC{double bond, long}O(OD)/2ΔC(OD) can be used to predict the extent of transmission via the hydrogen bond. Furthermore, deuterium isotope effects can be used to gauge possible tautomerism.