226404-83-7Relevant academic research and scientific papers
Development and validation of a reversed-phase HPLC method for determination of lesatropane and enantiomeric impurity
Yang, Li-Min,Xie, Yi-Fan,Gu, Zhou-Hui,Wang, Ai-Ling,Chen, Hong-Zhuan,Lu, Yang
body text, p. 581 - 584 (2012/01/02)
Lesatropane is a novel muscarinic receptor agonist and is currently being under preclinical development in China as a single enantiomer drug for the treatment of primary glaucoma. A reversed-phase chiral HPLC method for determination of lesatropane and enantiomeric impurity was developed. Enantiomeric separation of lesatropane from its enantiomer (desatropane) was achieved in normal-phase mode with Chiralpak AD-H and in reversed-phase mode with Chiralpak AS-RH. The conditions using a Chiralpak AS-RH column and mobile phase of K2HPO4-KH2PO4 (pH 7.0; 0.02 M)-acetonitrile (69:31, v/v) at a flow rate of 0.5 ml/min have been fully validated with satisfactory specificity, linearity, accuracy, and precision. The method was found to be suitable for the simultaneous quantitation of lesatropane and enantiomeric impurity desatropane. Copyright
