23305-68-2 Usage
General Description
MTT formazan is a chemical compound that is commonly used in cell viability assays to determine the metabolic activity and proliferation of cells. It is a yellow water-insoluble compound that is converted to a purple formazan product by the mitochondrial dehydrogenase enzymes of living cells. This conversion process is the basis for determining the viability of cells, as the amount of formazan produced is directly proportional to the metabolic activity of the cells. MTT formazan is typically used in assays such as the MTT assay or MTT-based cell proliferation assays to assess the effects of experimental treatments, drugs, or environmental factors on cell viability. Overall, MTT formazan is a crucial tool in the field of cell biology and is widely used for assessing cell viability in research and diagnostic applications.
Check Digit Verification of cas no
The CAS Registry Mumber 23305-68-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,3,3,0 and 5 respectively; the second part has 2 digits, 6 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 23305-68:
(7*2)+(6*3)+(5*3)+(4*0)+(3*5)+(2*6)+(1*8)=82
82 % 10 = 2
So 23305-68-2 is a valid CAS Registry Number.
InChI:InChI=1/C18H17N5S/c1-13-14(2)24-18(19-13)22-17(15-9-5-3-6-10-15)20-23(21-22)16-11-7-4-8-12-16/h3-12,21H,1-2H3
23305-68-2Relevant articles and documents
Modification of mtt assay for precision and repeatability and its mechanistic implication
Wang, Xu-Dong,Deng, Rui-Cheng,Liu, Yao,Li, Biao,Huang, Shen,Ouyang, Hui,Xiao, Zhu-Ping
, p. 8015 - 8018 (2014)
The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is an attractive method for antibiotics screening. However, a severe deviation will occur when a strong acidic sodium dodecyl sulfate is used as a lysis buffer in MTT assay and n
