24905-18-8Relevant academic research and scientific papers
Structural elucidation of three novel oligosaccharides from Kunlun Chrysanthemum flower tea and their bioactivities
Yu, Qian,Chen, Wei,Zhong, Jing,Qing, Degang,Yan, Chunyan
, (2021/02/16)
Coreopsis tinctoria is commonly called Kunlun Chrysanthemum and a plateau plant with tremendous commercial value in functional tea and medicinal applications. In folk medicine, Kunlun Chrysanthemum flower is often used as an adjunctive therapy for diabetes and Alzheimer's disease. To further explore the chemicals responsible for the health benefits of Kunlun Chrysanthemum flowers, three homogeneous oligosaccharides, CT70-1A, CT70-1B and CT70-2 were isolated, and their detailed structures were determined from chemical and spectral analyses. The three oligosaccharides were composed of glucose, mannose, galactose, and arabinose in different ratios. They showed dose-dependent α-amylase and α-glucosidase inhibitory effects. In addition, they showed NO production inhibitory activities in BV2 cells, with IC50 values of 0.23, 0.24 and 0.27 mM, respectively. Taken together, these results suggested that Kunlun Chrysanthemum oligosaccharides might ameliorate hyperglycemia and neuroinflammation, which could prevent the development of diseases such as type 2 diabetes and Alzheimer's disease. This study provides chemical and bioactive perspectives that support the consumption of Kunlun Chrysanthemum flower tea for health benefits.
Isolation, purification and structural characterization of a water-soluble polysaccharide HM41 from Halenia elliptica D. Don
Liu, Chun-Lan,Li, Yang,Xu, Gui-Yun,Li, Ya-Shuang
, p. 979 - 983 (2016/06/13)
A water-soluble polysaccharide, HM41, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM41 was composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal), glucose (Glc) with a molar ratio of 1.0:5.5:1.8:3.0:9.4:21. The average molecular weight of HM41 was approximately 1.17 × 104. Periodate oxidation, Smith degradation, methylation and GC, IR, NMR, XRD, GC-MS analysis were used for the structural analysis of HM41. Its main chain was composed mainly of β-(1 → 4)Gal, β-(1 → 4)Glc and β-(1 → 6)Glc. β-(1 → 4)Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues; (1 → 4)Glc had no branch; (1 → 6)Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of (1 → 3,6)-Rha, (1 → 4)/(1 → 5)-Ara, (1 → 4)/(1 → 5)-Xyl, (1 → 4,6)-Man and (1 → 2)-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM41 had strong scavenging activities in vitro hydroxyl. Overall, HM and HM41 may have potential applications in the antioxidants for medical and food industry.
Water-soluble polysaccharides from finger citron fruits (Citrus medica L. var. sarcodactylis)
He, Zhengchun,Liang, Fengjie,Zhang, Yuyang,Pan, Yuanjiang
, p. 100 - 104 (2014/04/03)
Four water-soluble polysaccharides, FCp-1, FCp-2, FCp-3, and FCp-4 were obtained from finger citron fruits (Citrus medica L. var. sarcodactylis) by hot-water extraction and ethanol precipitation, followed by routine separation procedure. Based on the calibration curve, molecular weights of them were estimated to be 113.9, 32.6, 140.3, and 177.1 kDa respectively. The acid hydrolysis, methylation, IR, GC-MS, and NMR experiments were used for composition analysis. FCp-1 was a heteropolysaccharide composed of arabinose, galactose, glucose, rhamnose, and xylose, with a molar ratio of 3.0:7.0:4.1:1.0:1.5. FCp-2 and FCp-4 were →4)-α-d-GalpA(1→ linking galacturonan differ in molecular weights. FCp-3 was a →6)-α-d-Glcp(1→ linking glucan. According to the results of in vitro assays, FCp-3 showed significantly and moderately enhancing capacities toward the proliferation of splenocytes and thymocytes respectively. Thus, FCp-3 or analogs may have further use as immunomodulatory agents.
Structural characterization and protective effect against murine sepsis of fucogalactans from Agaricus bisporus and Lactarius rufus
Ruthes, Andrea C.,Rattmann, Yanna D.,Carbonero, Elaine R.,Gorin, Philip A.J.,Iacomini, Marcello
experimental part, p. 1620 - 1627 (2012/04/11)
Fucogalactans from edible Agaricus bisporus (RFP-Ab) and wild Lactarius rufus (RFP-Lr) mushrooms were obtained on aqueous extraction followed by purification. RFP-Ab had Mw 43.8 × 104 g mol -1 and RFP-Lr Mw 1.4
Chemical analysis of an immunoenhancing water-soluble polysaccharide of an edible mushroom, Pleurotus florida blue variant
Dey, Biswajit,Bhunia, Sanjoy K.,Maity, Kankan K.,Patra, Sukesh,Mandal, Soumitra,Maiti, Swatilekha,Maiti, Tapas K.,Sikdar, Samir R.,Islam, Syed S.
body text, p. 2736 - 2741 (2011/02/21)
An immunoenhancing polysaccharide isolated from the aqueous extract of the fruit bodies of the mushroom, Pleurotus florida blue variant, was found to consist of d-glucose and d-galactose in a molar ratio of nearly 5:1. On the basis of sugar analysis, methylation analysis, periodate oxidation, Smith degradation, and NMR studies (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as: The molecule activated macrophages, splenocytes, and thymocytes.
Thermal Degradation of Glycosides, IV Degradation of Flavonoid Glycosides
Kim, Youn Chul,Higuchi, Ryuichi,Kitamura, Yoichi,Komori, Tetsuya
, p. 1285 - 1290 (2007/10/02)
By mere heating, the flavone (1, 7), flavanone (4, 10), and flavonol glycosides (13, 14) are converted into their aglycones and monoglycosides.The products of pyrolysis have been isolated by chromatography and their structures elucidated by chemical and spectroscopic methods.The compound derived from the sugar moiety in the pyrolysis of glucosides has been identified as levoglucosan (17).From the structures of some minor products (16, 18, 19), generated by the thermal degradation, a mechanism of their generation and the elucidation of the structures of rearranged ions (for example +), often generated in the FD-mass spectra of glycosides, are presented. Key Words: Degradation, thermal / Glycosides / Flavonoid glycosides / FDMS fragmentation / Carbohydrates
THE STRUCTURAL ELUCIDATION OF THE CAPSULAR POLYSACCHARIDE OF Klebsiella K68
Dutton, Guy G. S.,Parolis, Haralambos,Parolis, Lesley A. S.
, p. 249 - 260 (2007/10/02)
The structure of the capsular polysaccharide isolated from Klebsiella K68 bacteria has been elucidated by both chemical and spectroscopic methods.The structure is of the "3+1" type, similar in pattern to the structures of the polysaccharides of Klebsiella K11 and K57, having a single branch point on the uronic acid.The polysaccharide is shown to consist of the following repeating unit:
Structure of the Klebsiella type 10 capsular polysaccharide.
Sarkar,Roy
, p. 205 - 215 (2007/10/02)
The capsular polysaccharide from Klebsiella Type 10 was found to contain D-galactose, D-glucose, D-mannose, and D-glucuronic acid in the ratios 3:1:1:1. Acid hydrolysis of the polysaccharide gave one aldobiouronic acid, one aldotriouronic acid, one aldotetraouronic acid, and two neutral disaccharides the structures of which were established. The native and carboxyl-reduced polysaccharide have been subjected as appropriate to methylation analysis and Smith degradation. Degradation of the methylated polysaccharide with base established the identity of the sugar unit preceding the glucosyluronic acid residue. The anomeric configurations of the sugar residues were determined by oxidation of the acetylated native and carboxyl-reduced polysaccharides with chromium trioxide. Based on these studies, the hexasaccharide structure 1 has been assigned to the repeating unit of the K-10 polysaccharide.
