25023-94-3

Basic information

• Product Name: D-Galactose,O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-
• Synonyms: Galactose,O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-, D- (8CI); Neoagarooctaose
• CAS NO: 25023-94-3
• Molecular Formula: C48H74 O37
• Molecular Weight: 1243.08
• Mol File: 25023-94-3.mol
• Article Data: 2

Chemical Properties

• Appearance: /
• CAS DataBase Reference: D-Galactose,O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-(CAS DataBase Reference)
• NIST Chemistry Reference: D-Galactose,O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-(25023-94-3)
• EPA Substance Registry System: D-Galactose,O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-O-b-D-galactopyranosyl-(1®4)-O-3,6-anhydro-a-L-galactopyranosyl-(1®3)-(25023-94-3)

Safety Data

• Hazardous Substances Data: 25023-94-3(Hazardous Substances Data)

Usage

Description

Agarose is a polysaccharide found in red algae, typically Gelidium and Gracilaria. It is a strictly alternating polysaccharide of α-1,3 linked D-galactose and β-1,4 linked L-3,6 anhydrogalactose with occasional sulfation at position 6 of the anhydrogalactose residue. Agaro-oligosaccharides result from cleavage at galactose residues and neoagaro-oligosaccharides from cleavage at 3,6-anhydro residues. Neoagarooctaose is reported to have potential for novel cosmeceuticals.

Uses

Neoagarooctaose is a polymeric, bioactive oligosaccharide which is biosynthetically prepared from agarose, or, through hydrolysis of Gracilaria lemaneiformis agar.

Relevant articles and documents

Overexpression and characterization of a novel thermostable β-agarase YM01-3, from marine bacterium Catenovulum agarivorans YM01T

Genome sequencing of Catenovulum agarivorans YM01T reveals 15 open-reading frames (ORFs) encoding various agarases. In this study, extracellular proteins of YM01T were precipitated by ammonium sulfate and separated by one-dimensional gel electrophoresis. The results of in-gel agarase activity assay and mass spectrometry analysis revealed that the protein, YM01-3, was an agarase with the most evident agarolytic activity. Agarase YM01-3, encoded by the YM01-3 gene, consisted of 420 amino acids with a calculated molecular mass of 46.9 kDa and contained a glycoside hydrolase family 16 β-agarase module followed by a RICIN superfamily in the C-terminal region. The YM01-3 gene was cloned and expressed in Escherichia coli. The recombinant agarase, YM01-3, showed optimum activity at pH 6.0 and 60°C and had a Km of 3.78 mg mL-1 for agarose and a Vmax of 1.14 × 104 U mg-1. YM01-3 hydrolyzed the β-1,4-glycosidic linkages of agarose, yielding neoagarotetraose and neoagarohexaose as the main products. Notably, YM01-3 was stable below 50°C and retained 13% activity after incubation at 80°C for 1 h, characteristics much different from other agarases. The present study highlights a thermostable agarase with great potential application value in industrial production.