250581-89-6

Basic information

• Product Name: β-D-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine
• Synonyms: β-D-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine
• CAS NO: 250581-89-6
• Molecular Weight: 771.913
• Mol File: 250581-89-6.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: β-D-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine(CAS DataBase Reference)
• NIST Chemistry Reference: β-D-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine(250581-89-6)
• EPA Substance Registry System: β-D-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine(250581-89-6)

Safety Data

• Hazardous Substances Data: 250581-89-6(Hazardous Substances Data)

Upstream product

• 51549-27-0 β-D-5'-O-tert-butyldimethylsilyl-2'-deoxycytidine 
• 14470-28-1 mono-4-methoxytrityl chloride 
• 250581-87-4 β-D-5'-O-tert-butyldimethylsilyl-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine 

Downstream Products

• 250581-90-9 β-D-5'-O-(N-methylanthraniloyl)-N⁴,3'-O-bis(4-methoxytrityl)-2'-deoxycytidine 

Relevant articles and documents

Study of human deoxycytidine kinase binding properties using intrinsic fluorescence or new fluorescent ligands

A series of D- and L-enantiomers of cytidine or adenosine analogues and fluorescent N-methylanthraniloyl (MeNHBz) cytidine derivatives regiospecifically synthesized from cytidine or deoxycytidine were used to quantify the enantioselectivity of human deoxycytidine kinase (dCK) and to characterize its binding states. Both D- and L-enantiomers of these compounds induced significant bimodal quenchings of the intrinsic fluorescence of the enzyme. The ratios of dissociation constants Kd(D)/Kd(L) for the high affinity binding of non fluorescent cytidine derivatives were remarkably similar. β-D- and β-L-ATP gave monophasic titration curves and the enzyme displayed a preference for the natural enantiomer. This demonstrates the lack of enantioselectivity of dCK in the substrate binding steps of its mechanism. The results of other fluorescence experiments with MeNHBz-cytidine derivatives were consistent with an enzyme mechanism in which nucleotide binding precedes nucleoside binding.