253586-24-2Relevant academic research and scientific papers
Fast and reliable automated synthesis of RNA and partially 2'-O- protected precursors ('caged RNA') based on two navel, orthogonal 2'-O- protecting groups. Preliminary communication
Pitsch, Stefan,Weiss, Patrick A.,Wu, Xiaolin,Ackermann, Damian,Honegger, Thomas
, p. 1753 - 1761 (2007/10/03)
Two sets of RNA phosphoramidites, carrying the (fluoride-labile) 2'-O- [(triisopropylsilyl)oxy]methyl (=tom) group and the (photolabile) [(R)-1-(2- nitrophenyl)ethoxy]methyl (= (R)-npeom) group, were prepared (see 1-4 and 5- 8, resp.). The two protecting groups were completely orthogonal to each other. Three ribozyme-substrate constructs, protected each by a (R)-npeom group, were synthesized; on photolysis, efficient cleavage of this remaining protecting group occurred (Scheme 3). It could be demonstrated that the presence of one (R)-npeom group within a RNA strand has only a minor influence on the pairing properties of corresponding duplexes.
