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Usage

Uses

Used in Enzyme Inhibition Studies:
Adenylyl Imidodiphosphate is used as a competitive inhibitor for most ATP-dependent systems, particularly in the study of adenylate cyclase. It is effective because it is a good substrate for enzymes that hydrolyze between the αand β-phosphorus atom, yet it is resistant to enzymes cleaving between the βand γ-phosphorus atom.
Used in Fluorescence Assays:
Adenylyl Imidodiphosphate is used as a substrate in two-motor fluorescence assays, allowing researchers to study the interactions and dynamics of molecular motors in a controlled environment.
Used in Ion Channel Research:
Adenylyl Imidodiphosphate lithium salt hydrate is used in research to investigate the effects of monovalent cations (Li+, Na+, K+, NH4+, Rb+, and Cs+) on the solid and solution structures of nucleic acid components. This helps in understanding the role of these cations in various biological processes.
Used in Neuropharmacology:
Adenylyl Imidodiphosphate lithium salt hydrate has been used in studies to determine the effects of antidepressants on interferon-γ-induced microglial production of IL-6 and nitric oxide. This application aids in understanding the molecular mechanisms underlying the action of antidepressants and their potential impact on neuroinflammation.
Used in Pharmaceutical Research:
AMP-PNP can be used as a tool in the development of new drugs targeting adenylate cyclase or other ATP-dependent enzymes. By studying the interactions between these enzymes and AMP-PNP, researchers can gain insights into the design of more effective and selective therapeutic agents.

Biochem/physiol Actions

A non-hydrolyzable ATP analog. AMP-PNP competitively inhibits ATP-dependent enzyme systems, such as glutamine synthetase. It inhibits a number of enzymes which require the hydrolysis of ATP such as DNA topoisomerase II, SV40 large T-antigen helicase and a number of kinases. Blocks ATP-sensitive calcium-dependent potassium channels.