256953-68-1 Usage
Uses
Used in Molecular Biology Applications:
8-AMINO[1'-(N''-DANSYL)-4''-AMINOBUTYL]-5'-(1-AZIRIDINYL)-5'-DEOXYADENOSINE is used as a fluorescent cofactor reagent for the sequence-specific labeling of DNA methyltransferase from Thermus aquaticus (MTaql). This application is significant because it allows for the detection and analysis of specific DNA sequences, which can be crucial in understanding gene regulation and function.
Additionally, the reagent can be used to sequence-specifically label plasmid DNA in a MTaql-catalyzed reaction. This capability is valuable in various research and diagnostic applications, as it enables the precise identification and manipulation of specific DNA sequences.
Used in Genetic Research:
In the field of genetic research, 8-AMINO[1'-(N''-DANSYL)-4''-AMINOBUTYL]-5'-(1-AZIRIDINYL)-5'-DEOXYADENOSINE is used as a tool for studying the methylation patterns of DNA. Methylation is an important epigenetic modification that can influence gene expression and has been linked to various diseases when dysregulated. By using 8-AMINO[1'-(N''-DANSYL)-4''-AMINOBUTYL]-5'-(1-AZIRIDINYL)-5'-DEOXYADENOSINE as a fluorescent cofactor reagent, researchers can better understand the role of DNA methylation in gene regulation and its implications for health and disease.
Used in Diagnostic Applications:
8-AMINO[1'-(N''-DANSYL)-4''-AMINOBUTYL]-5'-(1-AZIRIDINYL)-5'-DEOXYADENOSINE can also be employed in the development of diagnostic tests for genetic disorders and diseases. The ability to sequence-specifically label DNA allows for the detection of specific genetic mutations or alterations that may be associated with certain conditions. This can lead to more accurate and targeted diagnostic approaches, ultimately benefiting patients and healthcare providers.
Check Digit Verification of cas no
The CAS Registry Mumber 256953-68-1 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,5,6,9,5 and 3 respectively; the second part has 2 digits, 6 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 256953-68:
(8*2)+(7*5)+(6*6)+(5*9)+(4*5)+(3*3)+(2*6)+(1*8)=181
181 % 10 = 1
So 256953-68-1 is a valid CAS Registry Number.
256953-68-1Relevant academic research and scientific papers
Pljevaljcic, Goran,Pignot, Marc,Weinhold, Elmar
, p. 3486 - 3492 (2003)
Sequence-specific labeling of DNA is of immense interest for analytical and functional studies of DNA. We present a novel approach for sequence-specific labeling of DNA using a newly designed fluorescent cofactor for the DNA methyltransferase from Thermus aquaticus (M·TaqI). Naturally, M·TaqI catalyzes the nucleophilic attack of the exocyclic amino group of adenine within the double-stranded 5′-TCGA-3′ DNA sequence onto the methyl group of the cofactor S-adenosyl-L-methionine (AdoMet) leading to methyl group transfer. The design of a new fluorescent cofactor for covalent labeling of DNA was based on three criteria: (1) Replacement of the methionine side chain of the natural cofactor AdoMet by an aziridinyl residue leads to M′TaqI-catalyzed nucleophilic ring opening and coupling of the whole nucleoside to DNA. (2) The adenosyl moiety is the molecular anchor for cofactor binding. (3) Attachment of a fluorophore via a flexible linker to the 8-position of the adenosyl moiety does not block cofactor binding. According to these criteria the new fluorescent cofactor 8-amino[1″-(N″-dansyl)-4″'-aminobutyl]-5′- (1-aziridinyl)-5′-deoxyadenosine (3) was synthesized. 3 binds about 4-fold better than the natural cofactor AdoMet to M′TaqI and is coupled with a short duplex oligodeoxynucleotide by M′TaqI. The identity of the expected modified nucleoside was verified by electrospray ionization mass spectrometry after enzymatic fragmentation of the product duplex. In addition, the new cofactor 3 was used to sequence-specifically label plasmid DNA in a M′TaqI-catalyzed reaction.
NEW COFACTORS FOR METHYLTRANSFERASES
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Page 19, (2010/02/05)
Aziridine derivatives of formula (I)are disclosed which can be used as cofactor for S-adenosyl-L-methionine-dependent methyltransferases.
