25709-81-3 Usage
Uses
Used in Biological and Chemical Research:
Dibromofluorescein serves as a valuable tool in biological and chemical research due to its fluorescent properties. It is used as a pH indicator, with its color shifting from yellow to red as the pH of a solution increases, allowing for the monitoring of pH changes in various experimental setups.
Used in Fluorescence Microscopy:
In fluorescence microscopy, dibromofluorescein is employed as a stain to visualize cellular structures and processes. Its fluorescence allows for the detection and analysis of specific components within cells, contributing to a better understanding of cellular functions and interactions.
Used in Flow Cytometry:
Dibromofluorescein is also utilized in flow cytometry, a technique used to measure the physical and chemical characteristics of cells. As a fluorescent marker, it enables the identification and sorting of different cell populations based on their specific properties, such as size, granularity, and surface markers.
Used as a Marker for Cell Viability and Membrane Integrity:
Dibromofluorescein is used to assess cell viability and membrane integrity in various assays. Its ability to penetrate compromised cell membranes allows researchers to differentiate between live and dead cells, providing valuable information on cell health and response to treatments.
Used in Antimicrobial Applications:
Dibromofluorescein has been investigated for its potential as an antibacterial and antifungal agent. Its ability to bind to microbial cells and disrupt their functions has shown promise in the development of new antimicrobial agents to combat drug-resistant infections.
Check Digit Verification of cas no
The CAS Registry Mumber 25709-81-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,5,7,0 and 9 respectively; the second part has 2 digits, 8 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 25709-81:
(7*2)+(6*5)+(5*7)+(4*0)+(3*9)+(2*8)+(1*1)=123
123 % 10 = 3
So 25709-81-3 is a valid CAS Registry Number.
