25988-63-0 Usage
Chemical Properties
white powder
Uses
Different sources of media describe the Uses of 25988-63-0 differently. You can refer to the following data:
1. Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. It has also been used as a transfection agent for efficient labeling and to facilitate endocytosis. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.
2. Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.
3. Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
Definition
ChEBI: The hydrobromide salt of poly(L-lysine).
General Description
Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.
Biochem/physiol Actions
Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.
Check Digit Verification of cas no
The CAS Registry Mumber 25988-63-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,5,9,8 and 8 respectively; the second part has 2 digits, 6 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 25988-63:
(7*2)+(6*5)+(5*9)+(4*8)+(3*8)+(2*6)+(1*3)=160
160 % 10 = 0
So 25988-63-0 is a valid CAS Registry Number.
InChI:InChI=1/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H