260397-83-9

Upstream product

• 14719-21-2 trifluoroacetyl-propargyl amine 
• 103882-09-3 N-Boc-4-I-Phe-OH 

Downstream Products

• 383372-63-2 N-{3-[4-(2-amino-2-carboxy-ethyl)-phenyl]-prop-2-ynyl}-6-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-isophthalamic acid 
• 383372-58-5 N-{3-[4-(2-tert-butoxycarbonylamino-2-carboxy-ethyl)-phenyl]-prop-2-ynyl}-6-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-isophthalamic acid 
• 260397-84-0 3-[4-(3-amino-prop-1-ynyl)-phenyl]-2-tert-butoxycarbonylamino-propionic acid 

Relevant academic research and scientific papers

Fluorescence resonance energy transfer terminators for DNA sequencing

A four-colour set of fluorescence resonance energy transfer dideoxy nucleotide terminators (10-13), have been synthesised using a rigid and linear tri-functional molecule (2), synthesised via Heck coupling reaction of t-Boc-L-4-iodophenylalanine (1) with N-TFA-propargylamine. Evaluation of the terminators in DNA sequencing reactions, in combination with Thermo Sequenase(TM) II DNA polymerase, demonstrated them to be excellent reagents for high-throughput DNA sequencing. (C) 2000 Elsevier Science Ltd.

Fluorescence resonance energy transfer dye nucleotide terminators: A new synthetic approach for high-throughout DNA sequencing

Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10-13) were designed, synthesized, and formulated with Thermo Sequenase II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transfer (ET) rigid and linear linker (2), required for the syntheses of energy transfer cassettes (6-9) was synthesized via Heck coupling reaction on t-Boc-L-4-iodo-phenylalanine (1) with N-TFA-propargylamine.