2610-05-1 Usage
Uses
Used in Fluorescence and Immunofluorescence Histochemistry:
CHICAGO SKY BLUE 6B is used as a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry. It helps in the examination of blood plasma permeation.
Used in Electrophysiological Recordings:
CHICAGO SKY BLUE 6B has also been used as a filling in glass pipette electrodes for electrophysiological recordings in the brain sections of rats.
Used in Textile Industry:
CHICAGO SKY BLUE 6B is used for dyeing cellulose fibers, such as cotton, viscose, and other cellulose fibers, silk, nylon, and rayon/nylon blend fabrics. It has excellent dyeing properties and good discharge properties, with supreme affinity at 60-80°C.
Used in Paper and Biological Stain:
CHICAGO SKY BLUE 6B can also be used for paper and biological staining applications.
Properties and Applications:
CHICAGO SKY BLUE 6B has good acid resistance, alkali resistance, light fastness, and soaping properties. It has ISO ratings of 5 for acid resistance, 4 for alkali resistance, 1 for light fastness, and 2 for soaping and water fading. The AATCC ratings are 5 for acid resistance, 1 for alkali resistance, 1 for light fastness, and 3-4 for soaping and water fading.
Preparation
3,3′-Dimethoxylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-1,3-disulfonic acid (2 Moore) coupled.
Air & Water Reactions
Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water.
Reactivity Profile
CHICAGO SKY BLUE 6B is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Cleavage of the diazo group by reducing agents may lead to benzidine production.
Fire Hazard
Flash point data for CHICAGO SKY BLUE 6B are not available; however, CHICAGO SKY BLUE 6B is probably combustible.
Biological Activity
Potent inhibitor of L-glutamate uptake into synaptic vesicles.
Biochem/physiol Actions
Chicago Sky Blue 6B is a large organic acid, structurally related to glutamate, that is a potent and efficient competitive inhibitor of vesicular glutamate uptake.
Safety Profile
An experimental
teratogen. Other experimental reproductive
effects. Mutation data reported. When
heated to decomposition it emits very toxic
fumes of NOx Na2O, and SOx
Standard
Acid Resistance
Fading
Stain
ISO
5
AATCC
5
Purification Methods
Free it from other electrolytes by adding aqueous sodium acetate to a boiling solution of the dye in distilled water. After standing, the salted-out dye is filtered on a Büchner funnel, the process being repeated several times. Finally, the precipitated dye is boiled several times with absolute EtOH to wash out any sodium acetate, then dried (as the sodium salt) at 105o. [McGregor et al. Trans Faraday Soc 58 1045 1962, Beilstein 16 II 259.]
Check Digit Verification of cas no
The CAS Registry Mumber 2610-05-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,6,1 and 0 respectively; the second part has 2 digits, 0 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 2610-05:
(6*2)+(5*6)+(4*1)+(3*0)+(2*0)+(1*5)=51
51 % 10 = 1
So 2610-05-1 is a valid CAS Registry Number.
InChI:InChI=1/C34H28N6O16S4.4Na/c1-55-23-11-15(3-7-19(23)37-39-21-9-5-17-25(57(43,44)45)13-27(59(49,50)51)31(35)29(17)33(21)41)16-4-8-20(24(12-16)56-2)38-40-22-10-6-18-26(58(46,47)48)14-28(60(52,53)54)32(36)30(18)34(22)42;;;;/h3-14,37-38H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-21-,40-22-;;;;
