2614-83-7 Usage
Description
2-Hydroxy-3-sulfanyl-propanoate, also known as 2-mercapto-3-hydroxypropionate, is an organic compound with a unique structure that features a hydroxyl and a sulfhydryl group. It is a versatile molecule with potential applications in various fields due to its functional groups that can participate in different chemical reactions and interactions.
Uses
Used in Pharmaceutical Industry:
2-Hydroxy-3-sulfanyl-propanoate is used as a building block for the synthesis of various pharmaceutical compounds. Its hydroxyl and sulfhydryl groups can be exploited to create new drugs with specific therapeutic properties.
Used in Biochemical Research:
In the field of biochemistry, 2-hydroxy-3-sulfanyl-propanoate is used as a reagent for the phosphorylation and acetylation of histone H2B. These post-translational modifications play a crucial role in regulating gene expression and are essential for understanding the molecular mechanisms underlying various cellular processes.
Used in Chemical Synthesis:
2-Hydroxy-3-sulfanyl-propanoate can be utilized as an intermediate in the synthesis of a wide range of organic compounds, including those with potential applications in materials science, agrochemicals, and specialty chemicals.
Used in Analytical Chemistry:
Due to its unique functional groups, 2-hydroxy-3-sulfanyl-propanoate can be employed as a chiral derivatizing agent for the analysis of enantiomers in analytical chemistry. This application can help in the development of more accurate and sensitive methods for detecting and quantifying chiral compounds.
Check Digit Verification of cas no
The CAS Registry Mumber 2614-83-7 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,6,1 and 4 respectively; the second part has 2 digits, 8 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 2614-83:
(6*2)+(5*6)+(4*1)+(3*4)+(2*8)+(1*3)=77
77 % 10 = 7
So 2614-83-7 is a valid CAS Registry Number.
InChI:InChI=1/C3H6O3S/c4-2(1-7)3(5)6/h2,4,7H,1H2,(H,5,6)
2614-83-7Relevant articles and documents
In vitro biosynthesis of 3-mercaptolactate by lactate dehydrogenases
Andree?en, Christina,Wolf, Natalie,Cramer, Benedikt,Humpf, Hans-Ulrich,Steinbüchel, Alexander
, p. 1 - 10 (2017/09/08)
3-Mercaptolactate (3ML) is an interesting mercapto compound with special regard to the biosynthesis of new polythioesters (PTEs). Unfortunately, this thioester analog of lactic acid is currently not commercially available. For this reason, we developed an in vitro biosynthesis pathway to convert cysteine to 3-mercaptopyruvate (3MPy), which is then rapidly and efficiently converted to 3ML by suitable lactate dehydrogenases (LDHs). As liver LDH from Rattus norvegicus (LDHRn) was previously described to Exhibit 3MPy reduction activity, in silico studies based on homology to LDHRn were performed and led to the identification of four potentially suitable bacterial LDH candidates from Escherichia coli (LDHEc), Corynebacterium glutamicum (LDHCg), Bacillus cereus (LDHBc) and Gloeobacter violaceus (LDHGv). After heterologous expression in E. coli followed by purification, the enzymes were assessed for their potential to reduce 3MPy to 3ML in comparison to LDHRn. With 3MPy, LDHs from E. coli, C. glutamicum and B. cereus showed no or only very low specific activities of 0.23 ± 0.1 U/mg (LDHCg) and 0.08 ± 0.2 U/mg (LDHBc), respectively. In contrast, LDHGv exhibited a remarkable specific activity of 63.6 ± 8.1 U/mg, being even twice as active as the R. norvegicus LDH. To verify LDH-catalyzed biosynthesis of 3ML we developed and optimized a detection method allowing qualitative analysis and quantification of 3MPy and 3ML by derivatization with Ellman's reagent and liquid chromatography-mass spectrometry. This study shows once more the impressive versatility of LDHs and presents a rapid and efficient biosynthesis process for 3ML, a biotechnologically interesting, yet hard-to-obtain, compound.
