263710-65-2Relevant academic research and scientific papers
Highly cooperative DNA dialkylation by the homodimer of imidazole - pyrrole diamide - CPI conjugate with vinyl linker
Tao, Zhi-Fu,Saito, Isao,Sugiyama, Hiroshi
, p. 1602 - 1608 (2000)
We synthesized new type of diamide - CPI conjugate possessing a vinyl linker, 7. Sequence-selective alkylation of double-stranded DNA by 7 was investigated by high-resolution denaturing gel electrophoresis using ～400 bp DNA fragments. Highly efficient alkylation predominantly occurs simultaneously at the purines of 5′-PyG(A/T)CPu-3′ site on both strands at a nanomolar concentration of 7. These results suggest that the homodimer of conjugate 7 dialkylates both strands according to Dervan's pairing rule together with a new mode of recognition in which the Im-vinyl linker (L) pair targets G/C base pairs. In addition to the major dialkylation sites, a minor alkylation site was also observed at 5′-GT(A/T)GC-3′. This alkylation can be explained by an analogous slipped homodimer recognition mode in which the L-L pair recognizes the A/T base pair. Efficient dialkylation by the homodimer of 7 was further confirmed using oligonucleotides (ODNs). HPLC analysis revealed that the conjugate 7 simultaneously alkylates GN3/AN3 of the target sequences on both strands of ODNs.
