2679-01-8 Usage
Uses
Used in Microbiology and Histology:
Methylene Green is used as a staining agent for the detection and visualization of microorganisms, such as bacteria and fungi, in microbiological samples. Its green color allows for easy identification and differentiation of these organisms from their surroundings. Additionally, it is used in histology as a counterstain to red or purple primary stains, enabling the clear visualization of cell nuclei and other cellular structures.
Used in Textile Industry:
Methylene Green is utilized as a dye in the textile industry, providing a dark blue to light green color to fabrics. Its solubility in water for green light blue and slightly soluble in ethanol for green light blue makes it suitable for various dyeing processes. The dye exhibits good light fastness, perspiration fastness, ironing fastness, and soaping properties, making it a preferred choice for染色 (dyeing) and 褪色 (fading) applications in the textile industry.
Used in Chemical Analysis:
Methylene Green is employed in chemical analysis as an indicator, particularly in titrations involving strong acids and bases. When dissolved in strong sulfuric acid, it appears green, while in diluted conditions, it turns light blue. Upon reaction with sodium hydroxide solution, the dye changes to purple, resulting in violet-black precipitation. This color change behavior makes it a useful tool for monitoring chemical reactions and determining endpoint titrations.
Preparation
commonly known as Methylene Green. (C.I.Basic Blue 9, C.I. 52015) in the first use of hydrochloric acid sodium nitrite processing, and then treated with nitric acid and zinc chloride double salt precipitation..
Standard
Light Fastness
Fading
Stain
B
2
A
5
Purification Methods
Crystallise the dye three times from water (18mL/g). [Beilstein 27 H 399.] The ZnCl2 double salt [6722-15-2] M 866.0 has the same CI.[Beilstein 27 III/IV 5157.]
Check Digit Verification of cas no
The CAS Registry Mumber 2679-01-8 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,6,7 and 9 respectively; the second part has 2 digits, 0 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 2679-01:
(6*2)+(5*6)+(4*7)+(3*9)+(2*0)+(1*1)=98
98 % 10 = 8
So 2679-01-8 is a valid CAS Registry Number.
InChI:InChI=1/C16H17N4O2S.ClH/c1-18(2)10-5-6-11-14(9-10)23-16-12(17-11)7-8-13(19(3)4)15(16)20(21)22;/h5-9H,1-4H3;1H/q+1;/p-1
