273383-41-8Relevant academic research and scientific papers
Visible diode laser induced fluorescence detection in liquid chromatography after precolumn derivatization of thiols
Mank, Arjan J. G.,Molenaar, Ernst J.,Lingeman, Henk,Gooijer, Cees,Brinkman, Udo A. Th.,Velthorst, Nel H.
, p. 2197 - 2203 (1993)
New cyanine labels containing only a single active group for the chemical derivatization of thiols were synthesized in order to apply visible diode laser induced fluorescence (DIO-LIF) detection in conventional-size liquid chromatography (LC). 2-Mercaptobenzothiazole (MBT) was selected as test compound. The most appropriate label was CY5.4a-IA, a sulfonated dicarbocyanine with a iodoacetamide reactive group. The detection limit of labeled MBT in the LC-DIO-LIF system was 8 X 10-12 M. The detection limit of MBT was 1 × 10-9 M; lower concentrations are not labeled quantitatively. The injection repeatability was 1.0% (n = 6), the reaction repeatability was 3.0% (n = 6), and linearity was observed from 2.5 × 10-9 to 1.0 × 10-5 M. As an example, MBT-spiked river water and urine samples were derivatized with CY5.4a-IA and analyzed by LC-DIO-LIF. Hardly any interference was found. The method compares very favorably with existing techniques.
