27580-13-8 Usage
Uses
Used in Research and Diagnostic Applications:
NAPHTHOL AS-D PHOSPHATE is used as a fluorescent stain for detecting and quantifying alkaline phosphatase activity in enzyme-linked immunosorbent assays (ELISAs). It is employed for its high sensitivity and stability, allowing for the accurate identification of specific enzymes and markers in biological samples.
Used in Combination with Other Substrates:
NAPHTHOL AS-D PHOSPHATE is used in conjunction with other substrates to achieve specific detection of alkaline phosphatase enzyme. This combination enhances the accuracy and reliability of the detection process, making it a valuable tool for research and diagnostic purposes.
Used in Biological and Medical Applications:
In the biological and medical fields, NAPHTHOL AS-D PHOSPHATE is used as a fluorescent stain for various applications, including the detection of certain enzymes and markers in biological samples. Its high sensitivity and stability make it an essential reagent for these applications.
Check Digit Verification of cas no
The CAS Registry Mumber 27580-13-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,7,5,8 and 0 respectively; the second part has 2 digits, 1 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 27580-13:
(7*2)+(6*7)+(5*5)+(4*8)+(3*0)+(2*1)+(1*3)=118
118 % 10 = 8
So 27580-13-8 is a valid CAS Registry Number.
InChI:InChI=1/C18H16NO5P/c1-12-6-2-5-9-16(12)19-18(20)15-10-13-7-3-4-8-14(13)11-17(15)24-25(21,22)23/h2-11H,1H3,(H,19,20)(H2,21,22,23)
27580-13-8Relevant academic research and scientific papers
Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids
Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi
, p. 1347 - 1353 (2007/10/02)
A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.
