287731-90-2Relevant articles and documents
Highly efficient synthesis of β(1 → 4)-oligo- and -polysaccharides using a mutant cellulase
Fort, Sebastien,Boyer, Viviane,Greffe, Lionel,Davies, Gideon J.,Moroz, Olga,Christiansen, Lars,Schuelein, Martin,Cottaz, Sylvain,Driguez, Hugues
, p. 5429 - 5437 (2000)
This report describes an efficient chemoenzymatic synthesis of a variety of regioselectively modified β(1→4)-oligo, and -polysaccharides. This successful approach was based on: (i) the use of a 'glycosynthase' which is a Glu-197-Ala nucleophile mutant of the retaining cellulase endoglucanase I (Cel7B) from Humicola insolens and (ii) the rational design of modified acceptor and donor molecules through a careful examination of information given by the X-ray structures of wild type and mutated enzymes. The mutant was able to catalyze, in high yield, the regio- and stereoselective glycosylation of α-glycobiosyl fluorides both unsubstituted and modified with various mono- and disaccharide acceptors, as well as the polymerization of these donors through a single-step inverting mechanism.