296776-06-2 Usage
General Description
4-Methylumbelliferyl 3-O-(α-L-fucopyranosyl)-β-D-galactopyranoside is a synthetic fluorogenic substrate commonly used in biochemical assays to detect and quantify the activity of α-L-fucosidase enzymes. The molecule consists of a 4-methylumbelliferyl fluorophore linked to a disaccharide composed of α-L-fucose and β-D-galactose. When the substrate is cleaved by α-L-fucosidase, the fluorophore is released and emits a blue fluorescence, allowing for the convenient and sensitive measurement of enzyme activity. 4-Methylumbelliferyl 3-O-(a-L-fucopyranosyl)-b-D-galactopyranoside finds applications in various research fields, such as glycobiology, enzymology, and drug discovery, where the accurate assessment of α-L-fucosidase activity is required.
Check Digit Verification of cas no
The CAS Registry Mumber 296776-06-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,9,6,7,7 and 6 respectively; the second part has 2 digits, 0 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 296776-06:
(8*2)+(7*9)+(6*6)+(5*7)+(4*7)+(3*6)+(2*0)+(1*6)=202
202 % 10 = 2
So 296776-06-2 is a valid CAS Registry Number.
InChI:InChI=1/C22H28O12/c1-8-5-14(24)32-12-6-10(3-4-11(8)12)31-22-19(29)20(16(26)13(7-23)33-22)34-21-18(28)17(27)15(25)9(2)30-21/h3-6,9,13,15-23,25-29H,7H2,1-2H3
296776-06-2Relevant articles and documents
Stereoselective synthesis of α-L-Fucp-(1,2)- and -(1,3)-β-D-Galp(1)-4-methylumbelliferone using glycosyl donor substituted by propane-1,3-diyl phosphate as leaving group
Vankayalapati, Hariprasad,Singh, Gurdial
, p. 2187 - 2193 (2000)
Stereoselective synthesis of α-L-Fucp-(1,2)- and -(1,3)-β-D-Galp(1)-4-methyllumbelliferone was accomplished using glycosyl donor substituted by propane-1,3-diyl phosphate as leaving group. The α-stereochemistry for the anomeric centre was assigned on the basis of the observed 13C-H coupling constant of 165.4 Hz for the bond. The results suggested that the bond-forming reaction intermediate was formed through participation of the second carbon acetoxy function or by an SN2-like process.