3012-71-3

Basic information

• Product Name: 6-FITC
• Synonyms: 2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-4-ISOTHIOCYANATO-BENZOIC ACID;6-FITC;6-ISOTHIOCYANATO-FLUORESCEIN;FITC ISOMER II;FITC 'ISOMER II';FITC;FLUORESCEIN 6-ISOTHIOCYANATE, ISOMER 2;FLUORESCEIN ISOTHIOCYANATE (ISOMER II)
• CAS NO: 3012-71-3
• Molecular Formula: C₂₁H₁₁NO₅S
• Molecular Weight: 389.38
• EINECS: 248-207-7
• Product Categories: Fluorescent Labels and Indicators;Fluorescent Labels & Indicators
• Mol File: 3012-71-3.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 719.1°C at 760 mmHg
• Flash Point: 388.7°C
• Appearance: /
• Density: 1.54g/cm³
• Vapor Pressure: 2.28E-21mmHg at 25°C
• Refractive Index: 1.753
• Storage Temp.: 2-8°C
• CAS DataBase Reference: 6-FITC(CAS DataBase Reference)
• NIST Chemistry Reference: 6-FITC(3012-71-3)
• EPA Substance Registry System: 6-FITC(3012-71-3)

Safety Data

• Hazard Codes: Xi
• Statements: 36-42/43
• Safety Statements: 26-45-36/37-22
• WGK Germany: 3
• Hazardous Substances Data: 3012-71-3(Hazardous Substances Data)

Usage

Chemical Properties

Orange powder

Uses

6-FITC is a fluorescent labeling reagent for proteins,it is Used in the fluorescent antibody technique for rapid identification of pathogens.

General Description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The Fc fragment contains the CH2 and CH3 domains of the antibody molecule. Pepsin cleaves the carboxy-terminal side of the disulfide bonds in the general region of the antibody and this gives rise to the F(ab′)2 fragment. The two antigen-binding arms of the antibody are linked in this fragment. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants.

InChI:InChI=1/C21H11NO5S/c23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h1-9,23-24H