303-01-5

Basic information

• Product Name: hydroxydione
• Synonyms: hydroxydione;17β-(Hydroxyacetyl)-5β-androstane-3-one;5beta-dihydrodeoxycorticosterone
• CAS NO: 303-01-5
• Molecular Formula: C₂₁H₃₂O₃
• Molecular Weight: 332.47698
• Mol File: 303-01-5.mol
• Article Data: 1

Chemical Properties

• Boiling Point: 468.6°C at 760 mmHg
• Flash Point: 251.3°C
• Appearance: /
• Density: 1.11g/cm³
• Vapor Pressure: 9.42E-11mmHg at 25°C
• Refractive Index: 1.531
• CAS DataBase Reference: hydroxydione(CAS DataBase Reference)
• NIST Chemistry Reference: hydroxydione(303-01-5)
• EPA Substance Registry System: hydroxydione(303-01-5)

Safety Data

• Hazardous Substances Data: 303-01-5(Hazardous Substances Data)

Usage

Uses

Hydroxydione is one of the chemicals studied for acute oral toxicity, repellency and hazard potential to species of wild and domestic birds.

Definition

ChEBI: A 3-oxo steroid with 5beta-configuration formed from 11-deoxycorticosterone by reduction across the C4-C5 double bond.

InChI:InChI=1/C21H32O3/c1-20-9-7-14(23)11-13(20)3-4-15-16-5-6-18(19(24)12-22)21(16,2)10-8-17(15)20/h13,15-18,22H,3-12H2,1-2H3/t13-,15+,16+,17+,18-,20+,21+/m1/s1

Upstream product

• 64-85-7 21-Hydroxyprogesterone 
• 53-57-6 NADPH 

Downstream Products

• 80-96-6 succinic acid mono-(3,20-dioxo-5β-pregnan-21-yl ester) 
• 65310-34-1 3β,20,21-Trihydroxy-5β-pregnan 
• 2041-77-2 3α,20,21-Trihydroxy-5β-pregnan 
• 567-03-3 tetrahydrodeoxycorticosterone 

Relevant articles and documents

Molecular cloning and heterologous expression of progesterone 5β-reductase from Digitalis lanata Ehrh.

A full-length cDNA clone that encodes progesterone 5β-reductase (5β-POR) was isolated from Digitalis lanata leaves. The reading frame of the 5β-POR gene is 1170 nucleotides corresponding to 389 amino acids. For expression, a Sph1/Sal1 5β-POR fragment was cloned into the pQE vector and was transformed into Escherichia coli strain M15[pREP4]. The recombinant gene was functionally expressed and the recombinant enzyme was characterized. The Km and vmax values for the putative natural substrate progesterone were calculated to be 0.120 mM and 45 nkat mg-1 protein, respectively. Only 5β-pregnane-3,20-dione but not its α-isomer was formed when progesterone was used as the substrate. Kinetic constants for cortisol, cortexone, 4-androstene-3,17-dione and NADPH were also determined. The molecular organization of the 5β-POR gene in D. lanata was determined by Southern blot analysis. The 5β-POR is highly conserved within the genus Digitalis and the respective genes and proteins share considerable homology to putative progesterone reductases from other plant species.