3129-48-4 Usage
Chemical structure
A proline amino acid derivative with a 2,4-dinitrophenyl group attached to the nitrogen atom.
Application
Used as a derivatizing agent in chromatography and mass spectrometry for the detection and quantification of amino acids.
Reaction mechanism
Capable of reacting with amino acids through a nucleophilic substitution reaction to form stable derivatives.
Specificity
Specific for proline, making it a useful tool for the analysis of proline-containing peptides and proteins.
UV absorbance
The 2,4-dinitrophenyl group imparts a high degree of UV absorbance, making it suitable for use in HPLC and LC-MS methods for amino acid analysis.
Derivatization
Enhances the detection and quantification of amino acids by forming stable derivatives that are easily detectable.
Analytical technique
Widely used in various analytical techniques such as high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) for amino acid analysis.
Structural modification
The presence of the 2,4-dinitrophenyl group allows for the differentiation of proline from other amino acids in a sample.
Sensitivity
The high UV absorbance of the 2,4-dinitrophenyl group increases the sensitivity of the detection method, allowing for the analysis of even small amounts of proline-containing compounds.
Versatility
Can be used in various research and diagnostic applications, including the study of protein structure, function, and interactions, as well as the identification and quantification of proline in biological samples.
Check Digit Verification of cas no
The CAS Registry Mumber 3129-48-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,1,2 and 9 respectively; the second part has 2 digits, 4 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 3129-48:
(6*3)+(5*1)+(4*2)+(3*9)+(2*4)+(1*8)=74
74 % 10 = 4
So 3129-48-4 is a valid CAS Registry Number.
