313223-04-0 Usage
Description
3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO)-&, also known as 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, is a zwitterionic detergent primarily used for the solubilization of membrane proteins. It possesses both positive and negative charges within its molecular structure, allowing it to interact with membrane proteins and facilitate their solubilization in aqueous solutions.
Uses
Used in Biochemistry and Molecular Biology:
3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO)-& is used as a solubilization agent for membrane proteins for the purpose of studying their structure, function, and interactions with other molecules. This is crucial in understanding various biological processes and developing targeted therapies for diseases.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, 3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO)-& is used as a detergent for the purification and stabilization of membrane proteins, which are often challenging to work with due to their hydrophobic nature. This allows for the development of drugs that target these proteins, potentially leading to novel treatments for various diseases.
Used in Structural Biology:
3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO)-& is employed as a detergent in structural biology to facilitate the crystallization of membrane proteins for X-ray crystallography studies. This helps researchers determine the three-dimensional structure of these proteins, which is essential for understanding their function and designing drugs that can modulate their activity.
Used in Drug Delivery Systems:
In drug delivery systems, 3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO)-& can be used as a component of nanoparticles or liposomes to encapsulate and protect membrane protein-based drugs, enhancing their stability, bioavailability, and targeted delivery to specific cells or tissues.
Purification Methods
Triturate this zwitterionic detergent (~50g) with MeOH (200ml), then triturate it with Me2CO (500mL), collect it by vacuum filtration and dry it thoroughly at ~20o. Recrystallise it at 0o from absolute MeOH followed by drying in vacuo to constant weight. It should give one spot with RF 0.32 by TLC on silica gel G in 95% MeOH/5% NH4OH and visualized with iodine vapour; the tertiary amine precursor has RF 0.40. It has low UV absorption (1% in H2O: A280nm is 0.029 and A260nm is 0.035), and the CMC (critical micellar concentration) is 8mM. It is soluble in H2O, MeOH, Me2SO but insoluble in Me2CO, MeCN, hexane, toluene and xylene. [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.] CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate betaine) [82473-24-3] M 630.89, m 184-196o, [ ] D +19o (c 2, H2O), pKEst (1) ~<2 (acidic), pKEst(2) ~9 (basic). Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.]
Check Digit Verification of cas no
The CAS Registry Mumber 313223-04-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 3,1,3,2,2 and 3 respectively; the second part has 2 digits, 0 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 313223-04:
(8*3)+(7*1)+(6*3)+(5*2)+(4*2)+(3*3)+(2*0)+(1*4)=80
80 % 10 = 0
So 313223-04-0 is a valid CAS Registry Number.
InChI:InChI=1/C32H58N2O7S.2H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);2*1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30?,31+,32-;;/m1../s1
