31641-72-2

Upstream product

• 24173-36-2 4-azidobenzaldehyde 
• 603-35-0 triphenylphosphine 

Downstream Products

• 30980-77-9 ethyl 3-(4-aminophenyl)-2-cyanoacrylate 
• 24611-27-6 (4-Triphenylphosphoranylidenamino-benzyliden)-malononitril 
• 28435-37-2 C₃₀H₂₅N₂O₂P 
• 42457-70-5 3-ethyl-2-thioxo-5-[4-(triphenyl-λ⁵-phosphanylideneamino)-benzylidene]-thiazolidin-4-one 

Relevant academic research and scientific papers

A photoactivatable push-pull fluorophore for single-molecule imaging in live cells

We have reengineered a red-emitting dicyanomethylenedihydrofuran push-pull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission. Copyright

Fluorogenic compounds converted to fluorophores by photochemical or chemical means and their use in biological systems

Fluorophores derived from photoactivatable azide-pi-acceptor fluorogens or from a thermal reaction of an azide-pi-acceptor fluorogen with an alkene or alkyne are disclosed. Fluorophores derived from a thermal reaction of an alkyne-pi-acceptor fluorogen with an azide are also disclosed. The fluorophores can readily be activated by light and can be used to label a biomolecule and imaged on a single-molecule level in living cells.