329321-59-7

Upstream product

• 329321-53-1 1-(2-deoxy-β-D-erythro-pentofuranosyl)-1,5-dihydro-6H-pyrazolo[3,4-d]pyrimidin-6-one 
• 266317-61-7 6-amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1H-pyrazolo[3,4-d]pyrimidine 
• 83-01-2 diphenylcarbamic chloride 
• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 329321-57-5 6-(N,N-diphenylcarbamoyl)oxy-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1H-pyrazolo[3,4-d]pyrimidine 

Relevant academic research and scientific papers

Fluorescence properties and base pair stability of oligonucleotides containing 8-aza-7-deaza-2′-deoxyisoinosine or 2′-deoxyisoinosine

The fluorescence and the base pairing properties of 8-aza-7-deaza-2′-deoxyisoinosine (1) are described and compared with those of 2′-deoxyisoinosine (2). The corresponding phosphoramidites (11, 12) are synthesized using the diphenylcarbamoyl (DPC) residue for the 2-oxo group protection. The nucleosides 1 and 2 base pair with 2′-deoxy-5-methylisocytidine in DNA duplexes with antiparallel chain orientation and with 2′-deoxycytidine in a parallel DNA. These base pairs are less stable than the canonical dA-dT pair and that of 2′-deoxyinosine (4) with 2′-deoxycytidine. The fluorescence of the nucleosides 1 and 2 is quenched (～95%) in duplex DNA. The residual fluorescence is used to determine the Tm-values, which are found to be the same as determined UV-spectrophotometrically.