330203-79-7Relevant academic research and scientific papers
Enzymatic synthesis of tumor-associated carbohydrate antigen globo-H hexasaccharide
Su, Doris M.,Eguchi, Hironobu,Yi, Wen,Li, Lei,Wang, Peng G.,Xia, Chengfeng
, p. 1009 - 1012 (2008)
We report the enzymatic synthesis of an important tumor-associated carbohydrate antigen, Globo-H hexasaccharide. Starting with Lac-OBn as the initial acceptor, this approach employs three glycosyltransferases: LgtC, an α1,4-galactosyltransferase; LgtD, a bifunctional β1,3- galactosyl/β1,3-N-acetylgalactosaminyltransferase; and WbsJ, an α1,2-fucosyltransferase. In addition, two epimerases, GalE and WbgU, were also employed for the generation of more expensive sugar nucleotides, UDP-Gal and UDP-GalNAc, from their corresponding inexpensive C4 epimers. This study represents a facile enzymatic synthesis of the Globo-H antigen.
