33224-03-2Relevant academic research and scientific papers
The novel assay method for nicotine metabolism to cotinine using high performance liquid chromatography
Miyazawa, Mitsuo,Kawauchi, Yumi,Okuno, Yoshiharu,Oda, Yoshimitsu
, p. 295 - 297 (2011)
Nicotine is the primary psychoactive component in tobacco. It is taken into the body by tobacco smoking, and mainly metabolized to cotinine in the hepatic cytochrme P450 (CYP) 2A6. The objective of this study was to develop a sensitive method for the determination of nicotine metabolism to cotinine using HPLC. The internal standard, trans-4′-carboxycotinine methyl ester was synthesized with a simple method. The nicotine and cotinine were separated completely and detected by C18 5- μm analytical column (L-column Octa decyl silyl (ODS), 150mmx4.6 mm i.d.) equipped with a C18 5- μm guard column (L-column ODS, 10mmx4.6 mm i.d.) and ultraviolet detection at 260 nm. The detection limit of the assay was 0.05 μM for cotinine (n=5, R.S.D) and 0.1 μM for nicotine. Thus the present results provided a sensitive and useful method for the determination of nicotine metabolism catalyzed by CYP2A6.
