Z-Leu-O3NP, also known as Z-leucine-nitrophenyl phosphate, is a synthetic chemical compound that consists of a leucine residue (Z-protected) linked to a nitrophenyl phosphate group. Z-Leu-O3NP is often used in biochemical research, particularly in the study of enzymes such as proteases and phosphatases. The Z-group (benzyloxycarbonyl) is a protecting group that temporarily blocks the amino group of the leucine, preventing unwanted side reactions. The nitrophenyl phosphate group serves as a reporter or a leaving group, which can be easily detected due to its yellow color and its ability to change color upon hydrolysis. This makes Z-Leu-O3NP a useful substrate for assaying enzyme activity and studying enzyme kinetics, as the release of the nitrophenol can be monitored spectrophotometrically, providing a quantitative measure of enzyme activity.
The CAS Registry Mumber 33457-79-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,3,4,5 and 7 respectively; the second part has 2 digits, 7 and 9 respectively. Calculate Digit Verification of CAS Registry Number 33457-79: (7*3)+(6*3)+(5*4)+(4*5)+(3*7)+(2*7)+(1*9)=123 123 % 10 = 3 So 33457-79-3 is a valid CAS Registry Number.