33985-07-8Relevant academic research and scientific papers
Supramolecular Inclusion Complexes of a Star Polymer Containing Cholesterol End-Capped Poly(e-caprolactone) Arms with b-Cyclodextrin
Doganci, Erdinc,Gorur, Mesut,Uyanik, Cavit,Yilmaz, Faruk
, p. 3406 - 3420 (2014)
A novel hexa-armed and star-shaped polymer containing cholesterol end-capped poly(e-caprolactone) arms emanating from a phosphazene core (N3P3-(PCL-Chol)6) was ynthesized by a combination of ring-opening polymerization and "click" chemistry techniques. For this purpose, the terminal AOH groups of the synthesized precursor (N3P3-(PCL-OH)6) were converted into Chol through a series of reaction. Both 3P3-(PCL-OH)6 and N3P3-(PCL-Chol)6 were then employed in the preparation of supramolecular inclusion complexes (ICs) with b-cyclodextrin (b-CD). The latter formed ICs with b-CD in higher yield. The hostguest stoichiometry (e-CL:b-CD, mol:- mol) in the ICs of N3P3-(PCL-Chol)6 was found to be 1.2. The formation of supramolecular ICs of N3P3-(PCL-Chol)6 with b-CD was confirmed by using Fourier transform infrared (FTIR) and 1H nuclear magnetic resonance (NMR) spectroscopic methods, wide-angle X-ray diffraction (WAXD), and thermal analysis techniques. WAXD data showed that the obtained ICs with N3P3-(PCL-Chol)6 had a channel-type crystalline structure, indicating the suppression of the original crystallization of N3P3- (PCL-Chol)6 in b-CD cavities. Moreover, the thermal stabilities of ICs were found to be higher than those of the free star polymer and b-CD. Furthermore, the surface properties of N3P3- (PCL-Chol)6 and its ICs with b-CD were investigated by static contact angle measurements. The obtained results proved that the wettability of N3P3-(PCL-Chol)6 successfully increased with the formation of its ICs with b-CD.
'Click' synthesized sterol-based cationic lipids as gene carriers, and the effect of skeletons and headgroups on gene delivery
Sheng, Ruilong,Luo, Ting,Li, Hui,Sun, Jingjing,Wang, Zhao,Cao, Amin
, p. 6366 - 6377 (2013/10/22)
In this work, we have successfully prepared a series of new sterol-based cationic lipids (1-4) via an efficient 'Click' chemistry approach. The pDNA binding affinity of these lipids was examined by EB displacement and agarose-gel retardant assay. The average particle sizes and surface charges of the sterol-based cationic lipids/pDNA lipoplexes were analyzed by dynamic laser light scattering instrument (DLS), and the morphologies of the lipoplexes were observed by atomic force microscopy (AFM). The cytotoxicity of the lipids were examined by MTT and LDH assay, and the gene transfection efficiencies of these lipid carriers were investigated by luciferase gene transfection assay in various cell lines. In addition, the intracellular uptake and trafficking/localization behavior of the Cy3-DNA loaded lipoplexes were preliminarily studied by fluorescence microscopy. The results demonstrated that the pDNA loading capacity, lipoplex particle size, zeta potential and morphology of the sterol lipids/pDNA lipoplexes depended largely on the molecular structure factors including sterol-skeletons and headgroups. Furthermore, the sterol-based lipids showed quite different cytotoxicity and gene transfection efficacy in A549 and HeLa cells. Interestingly, it was found that the cholesterol-bearing lipids 1 and 2 showed 7-104 times higher transfection capability than their lithocholate-bearing counterparts 3 and 4 in A549 and HeLa cell lines, suggested that the gene transfection capacity strongly relied on the structure of sterol skeletons. Moreover, the study on the structure-activity relationships of these sterol-based cationic lipid gene carriers provided a possible approach for developing low cytotoxic and high efficient lipid gene carriers by selecting suitable sterol hydrophobes and cationic headgroups.
Synthesis, characterization, and the photochromic, luminescence, metallogelation and liquid-crystalline properties of multifunctional platinum(II) bipyridine complexes
Li, Yongguang,Tam, Anthony Yiu-Yan,Wong, Keith Man-Chung,Li, Wen,Wu, Lixin,Yam, Vivian Wing-Wah
experimental part, p. 8048 - 8059 (2011/09/14)
A series of multifunctional platinum(II) bipyridine complexes were designed, synthesized, and characterized by 1H NMR, fast atom bombardment mass spectrometry (FAB-MS), and elemental analysis. Their electrochemical and photophysical properties
