343592-33-6Relevant academic research and scientific papers
Development of an enzyme mimic using self-selection
Gasparini, Giulio,Dal Molin, Marta,Corra, Stefano,Galzerano, Patrizia,Scrimin, Paolo,Prins, Leonard J.
, p. 122 - 126 (2013)
The development of a serine protease model using a self-selection protocol is described. The developed system mimics the regeneration step of an enzyme involved in covalent enzyme catalysis. A transition-state analogue of a transesterification reaction is used to self-select functional groups able to accelerate ester cleavage. It is shown that the insertion of a tertiary amine substituent flanking the reaction center reinforces transition-state stabilization by directing the reactive center towards the self-selected functionality. In addition, the tertiary amine activates a bland (solvent) nucleophile for attack on an ester bond similar to what occurs in a serine protease. A quantitative correspondence is observed between the amplification factors and catalytic activity, illustrating the potential of the dynamic covalent capture strategy to precisely detect and quantify weak noncovalent interactions.
