3526-97-4Relevant articles and documents
The effect of the 3'-terminal monophosphate group on the metal-ion-promoted hydrolysis of the phosphodiester bonds of short oligonucleotides
Kuusela, Satu,Azhayev, Alex,Guzaev, Andrei,Loennberg, Harri
, p. 1197 - 1202 (2007/10/02)
The effect of 3'-terminal monophosphate group on the metal-ion-promoted hydrolysis of the phosphodiester bonds of oligonucleotides has been studied.For this purpose, the rate constants for the hydrolysis of the following oligomers in the presence of Zn2+ and its 1,5,9-triazacyclododecane chelate, Zn2+aneN3, have been determined: (i) ApUpUp(2') and ApUpUp(3'), (ii) Up(Tp)nT (n = 0-4 and 7), (iii) Up(Tp)nTp (n = 0-4 and 7).The results obtained are used to propose a mechanism for the Zn2+ and Zn2+aneN3 promoted hydrolysis of polynucleotides.