35523-89-8 Usage
Chemical Properties
Crystalline solid; soluble in water and me thanol; forms dihydrochloride with HCl.
Uses
Different sources of media describe the Uses of 35523-89-8 differently. You can refer to the following data:
1. As a Schedule 1 controlled substance under the Chemical
Weapons Convention of 1993, the use of saxitoxin is extremely
limited outside of weaponized forms. Its use as a chemical
reagent in research is its other important use, as it has been
instrumental in experiments to elucidate the mechanisms
involved in sodium channels used in cellular communication.
2. Mussel poison; clam poison; paralytic shellfish poison; gonyaulax toxin. These poisonous shellfish have been connected to instances of toxic”red-tides” where the high concentration of algae discoloring the water were of the Gonyaulax genus.
Used as a too
3. Saxitoxin is an alkaloid of nonplant origin.It is the neurotoxic constituent of dinoflagel lates (Gonyaulax catenella and G. excavata)the so-called “red tide” found along the U.S.coast. Shellfish, clams, and scallops consumethis and become extremely poisonous forhuman consumption.
Definition
ChEBI: An alkaloid isolated from the marine dinoflagellates and cyanobacteria that causes paralytic shellfish poisoning.
Health Hazard
Saxitoxin is an extremely toxic substance.It binds to sodium channels and the blocksnerve membrane. In humans, ingestion ofSAXITOXIN can produce tingling andburning in the lip, tongue, face, and thewhole body within an hour. This is fol lowed by numbness, muscular incoordina tion, confusion, headache, and respiratoryfailure. Death may occur within 12 hours.LD50 value intraperitoneal (mice): 0.005mg/kgLD50 value oral (mice): 0.26 mg/kgIntravenous administration of 1 mL of1:2000 solution of prostigmine methylsulfatehas been reported to be effective againstsaxitoxin poisoning (Hodgson et al. 1988).
Environmental Fate
Dinoflagellates (flagellate protists, plankton) are primarily
responsible for the biosynthesis of saxitoxin, and bioaccumulation
tends to occur in several shellfish such as
mussels, clams, scallops, and cockles. Broth made from shellfish
can harbor saxitoxin as well due to its stability at normal
cooking temperatures. Consumption of the shellfish leads to
toxicity in humans, while consumption of other organisms in
which saxitoxin has accumulated (up the food chain, for
example) has historically affected other animals such as whales.
Data describing the environmental fate of saxitoxin is
extremely limited. There have been some studies investigating
the absorption/desorption capacities of various soils for the
compound, but more exhaustive studies are yet to be
reported.
Toxicity evaluation
Saxitoxin interrupts nerve transmissions by binding to voltagegated
sodium channels. Positively charged guanidinium
groups of saxitoxin interact with negatively charged carboxyl
groups at a site on the sodium channel (in a one-to-one ratio)
of neurons and muscle cells, resulting in blocked action
potentials and interrupted transmissions. The inactivation of
vasomotor nerves along with vascular smooth-muscle relaxation
follows and hypotension can additionally occur. When
muscles in the respiratory or cardiovascular system are affected,
death can result.
Intraperitoneal inoculation with STX extract in the freshwater
fish Hoplias malabaricus resulted in a variety of systemic
effects culminating in oxidative stress observed in the brain,
leading to lipid, protein, and DNA damage. Although the
exposure in this study was subchronic, apoptotic cellular
processes were implicated.
References
Schantz et al., J. Arner. Chern. Soc., 97, 1238 (1975)
Check Digit Verification of cas no
The CAS Registry Mumber 35523-89-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,5,5,2 and 3 respectively; the second part has 2 digits, 8 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 35523-89:
(7*3)+(6*5)+(5*5)+(4*2)+(3*3)+(2*8)+(1*9)=118
118 % 10 = 8
So 35523-89-8 is a valid CAS Registry Number.
InChI:InChI=1/C11H18N6O4/c12-7-4-1-2-10(19,20)11(4)6(16-8(13)17-11)5(15-7)3-21-9(14)18/h4-6,19-20H,1-3H2,(H2,12,15)(H2,14,18)(H3,13,16,17)/p+2/t4?,5?,6-,11+/m0/s1
35523-89-8Relevant academic research and scientific papers
Shimizu, Yuzuru,Hsu, Chien P.
, p. 314 - 315 (1981)
The structures of four paralytic shellfish poisons have been unequivocally established by interconversion and ultimate correlation with saxitoxin.
Lukowski, April L.,Ellinwood, Duncan C.,Hinze, Meagan E.,Deluca, Ryan J.,Du Bois,Hall, Sherwood,Narayan, Alison R. H.
, p. 11863 - 11869 (2018)
The remarkable degree of synthetic selectivity found in Nature is exemplified by the biosynthesis of paralytic shellfish toxins such as saxitoxin. The polycyclic core shared by saxitoxin and its relatives is assembled and subsequently elaborated through the installation of hydroxyl groups with exquisite precision that is not possible to replicate with traditional synthetic methods. Here, we report the identification of the enzymes that carry out a subset of C-H functionalizations involved in paralytic shellfish toxin biosynthesis. We have shown that three Rieske oxygenases mediate hydroxylation reactions with perfect site- and stereoselectivity. Specifically, the Rieske oxygenase SxtT is responsible for selective hydroxylation of a tricyclic precursor to the famous natural product saxitoxin, and a second Rieske oxygenase, GxtA, selectively hydroxylates saxitoxin to access the oxidation pattern present in gonyautoxin natural products. Unexpectedly, a third Rieske oxygenase, SxtH, does not hydroxylate tricyclic intermediates, but rather a linear substrate prior to tricycle formation, rewriting the biosynthetic route to paralytic shellfish toxins. Characterization of SxtT, SxtH, and GxtA is the first demonstration of enzymes carrying out C-H hydroxylation reactions in paralytic shellfish toxin biosynthesis. Additionally, the reactions of these oxygenases with a suite of saxitoxin-related molecules are reported, highlighting the substrate promiscuity of these catalysts and the potential for their application in the synthesis of natural and unnatural saxitoxin congeners.