357625-74-2

Upstream product

• 14470-28-1 mono-4-methoxytrityl chloride 
• 357625-73-1 {6-allyloxy-9-[5-(tert-butyl-dimethyl-silanyloxymethyl)-4-hydroxy-tetrahydro-furan-2-yl]-9H-purin-2-yl}-carbamic acid allyl ester 
• 18162-48-6 tert-butyldimethylsilyl chloride 
• 158391-96-9 O⁶-allyl-N²-[(allyloxy)carbonyl]-2'-deoxyguanosine 

Downstream Products

• 232919-02-7 O⁶-allyl-N²-allyloxycarbonyl-3'-O-p-methoxytrityl-2'-deoxyguanosine 5'-(allyl N,N-diisopropylphosphoramidite) 

Relevant academic research and scientific papers

A novel approach to oligodeoxyribonucleotides bearing phosphoric acid esters at the 3'-terminals via the phosphoramidite method with allyl protection: An efficient synthesis of base-labile nucleotide-amino acid and - peptide conjugates

A new method for synthesis of 3'-end-phosphorylated DNA oligomers via the phosphoramidite method with allyl protection has been developed. This method is particularly useful for the preparation of derivatives with base- labile structures such as oligoDNA-OPO(OH)OCH2CH(R)Z, in which Z is an electron-withdrawing function. For example, a oligonucleotide-amino acid conjugate, 5'TGTCGACACCCAATT3'-OPO(OH)OCH2CH(NH2)COOH, and a oligonucleotide-peptide conjugate, 5'TGTCGACACCCAATT3'- OPO(OH)OCH2CH(NH2)CONHCH2COOH, have been obtained in high purity.

A facile synthesis of 5′-end solid-anchored, 3′-end free oligodeoxyribonucleotides via the (5′→3′)-elongated phosphoramidite strategy

It is demonstrated that not only N2- but also O 6-protection of the guanine base is necessary for obtaining the oligodeoxyribonucleotides in high yields and at a high purity in the solid-phase synthesis via the (5′ → 3′)-chain elongated phosphoramidite approach.