364059-48-3Relevant academic research and scientific papers
New highly calcemic 1α,25-dihydroxy-19-norvitamin D3 compounds with modified side chain: 26,27-dihomo- and 26,27-dimethylene analogs in 20S-series
Sicinski, Rafal R.,Prahl, Jean M.,Smith, Connie M.,DeLuca, Hector F.
, p. 247 - 256 (2007/10/03)
New highly potent 2-substituted (20S)-1α,25-dihydroxy-19-norvitamin D3 analogs with elongated side chain were prepared by Wittig-Horner coupling of A-ring phosphine oxide with the corresponding protected (20S)-25-hydroxy Grundmann's ketones. Biologic evaluation in vitro and in vivo of the synthesized compounds was accomplished. All the synthesized vitamins possessing a 25-hydroxylated saturated side chain were slightly less active (3-5X) than 1α,25-dihydroxyvitamin D3 in binding to the porcine intestinal vitamin D receptor and significantly more potent (12-150X) in causing differentiation of HL-60 cells. In vivo, 2-methylene-26,27-dihomo and 2α-methyl-26,27-dimethylene analogs were at least 10 times more active, and 2α-methyl-26,27-dihomo compound at least 5 times more active than the vitamin D hormone both in stimulating intestinal calcium transport and bone calcium mobilization (serum calcium increase). It was also established that a 260 pmol dose of the corresponding 2β-methyl analogs had a similar effect on intestinal calcium transport and a much more pronounced effect on bone calcium mobilization as the same dose of 1α,25-dihydroxyvitamin D3. Copyright
