T4 Polynucleotide Kinase, also known as T4 PNK, is an enzyme derived from the T4 bacteriophage that plays a crucial role in molecular biology. It is highly specific and efficiently catalyzes the transfer of the gamma-phosphate from ATP to the 5' end of singleand double-stranded DNA and RNA. This enzyme is an essential tool for various molecular biology techniques due to its versatility and specificity.
37211-65-7
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Basic information
- Product Name: T4 POLYNUCLEOTIDE KINASE
- Synonyms: IUB: 2.7.1.78;EC 2.7.1.78;T4 PK;T4 POLYNUCLEATIDE KINASE;T4 POLYNUCLEOTIDE KINASE;POLYNUCLEOTIDE KINASE;POLYNUCLEOTIDE KINASE (T4);kinase(phosphorylating),polynucleotide5’-hydroxyl
- CAS NO:37211-65-7
- Molecular Formula:
- Molecular Weight: 0
- EINECS: 253-400-4
- Product Categories: N/A
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Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /buffered aqueous glycerol solution
- Density: N/A
- Refractive Index: N/A
- Storage Temp.: −20°C
- Solubility: N/A
- CAS DataBase Reference: T4 POLYNUCLEOTIDE KINASE(CAS DataBase Reference)
- NIST Chemistry Reference: T4 POLYNUCLEOTIDE KINASE(37211-65-7)
- EPA Substance Registry System: T4 POLYNUCLEOTIDE KINASE(37211-65-7)
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Safety Data
- Hazard Codes: N/A
- Statements: N/A
- Safety Statements: N/A
- WGK Germany: 3
- RTECS:
- F: 10
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 37211-65-7(Hazardous Substances Data)
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37211-65-7 Usage
Uses
Used in Molecular Biology Research:T4 Polynucleotide Kinase is used as a molecular biology tool for various applications, including:
1. Labeling DNA or RNA probes: T4 PNK is employed to add a phosphate group to the 5' end of probes, which allows for the attachment of labels such as fluorescent or radioactive markers. This is crucial for techniques like in situ hybridization and Northern blotting.
2. Creating primer molecules for DNA sequencing: The enzyme is used to phosphorylate primers, enabling them to be ligated to the target DNA sequence during the sequencing process. This step is essential for accurate and reliable sequencing results.
3. Generating synthetic DNA or RNA fragments with defined 5' phosphates: T4 PNK is utilized to add a phosphate group to the 5' end of synthetic DNA or RNA fragments, which is necessary for various applications such as site-directed mutagenesis, cloning, and the construction of plasmid vectors.
Check Digit Verification of cas no
The CAS Registry Mumber 37211-65-7 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,7,2,1 and 1 respectively; the second part has 2 digits, 6 and 5 respectively.Calculate Digit Verification of CAS Registry Number 37211-65:
(7*3)+(6*7)+(5*2)+(4*1)+(3*1)+(2*6)+(1*5)=97
97 % 10 = 7
So 37211-65-7 is a valid CAS Registry Number.
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T4 POLYNUCLEOTIDE KINASE
