376353-99-0

Basic information

• Product Name: 2-PHENYL-3-(4-NITROPHENYL)-5-METHYLTETRAZOLIUM CHLORIDE
• Synonyms: 3-(4-NITROPHENYL)-5-METHYL-2-PHENYLTETRAZOLIUM CHLORIDE;2-PHENYL-3-(4-NITROPHENYL)-5-METHYLTETRAZOLIUM CHLORIDE
• CAS NO: 376353-99-0
• Molecular Formula: C14H12ClN5O2
• Molecular Weight: 317.73
• EINECS: 932-453-3
• Product Categories: Tetrazolium Salts;Tetrazolium Salts & Formazans
• Mol File: 376353-99-0.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• CAS DataBase Reference: 2-PHENYL-3-(4-NITROPHENYL)-5-METHYLTETRAZOLIUM CHLORIDE(CAS DataBase Reference)
• NIST Chemistry Reference: 2-PHENYL-3-(4-NITROPHENYL)-5-METHYLTETRAZOLIUM CHLORIDE(376353-99-0)
• EPA Substance Registry System: 2-PHENYL-3-(4-NITROPHENYL)-5-METHYLTETRAZOLIUM CHLORIDE(376353-99-0)

Safety Data

• RIDADR: 1325
• HazardClass: 4.1
• PackingGroup: III
• Hazardous Substances Data: 376353-99-0(Hazardous Substances Data)

Usage

Description

2-PHENYL-3-(4-NITROPHENYL)-5-METHYLTETRAZOLIUM CHLORIDE, commonly referred to as MTT, is a yellow tetrazolium salt that serves as a vital reagent in cell biology and drug development research. It is water-soluble and is metabolized by active mitochondria in living cells into a purple formazan product, signifying cell viability. This characteristic makes MTT a crucial tool for assessing the metabolic activity of cells, which is indicative of their health and survival.

Uses

Used in Cell Viability Assays:
MTT is utilized as a viability indicator in cell viability assays for determining the metabolic activity of living cells. The conversion of MTT to formazan by succinate dehydrogenase in active mitochondria provides a quantitative measure of viable cells, making it an essential component in assessing cytotoxicity and cell health.
Used in Drug Development Studies:
In the pharmaceutical industry, MTT serves as a critical tool for drug screening and development. It helps researchers evaluate the potential cytotoxic effects of new compounds, ensuring the safety and efficacy of drug candidates before they proceed to clinical trials.
Used in Biochemical Assays:
MTT is employed in various biochemical assays to quantify cellular proliferation, enzyme activity, and overall cell viability. Its ability to provide a colorimetric readout of cell metabolic activity makes it a versatile reagent for a wide range of biological samples and research applications.
Used in Research Applications:
MTT is used as a research tool in various scientific fields, including cell biology, toxicology, and pharmacology. Its ability to assess cell viability and metabolic activity makes it invaluable for studying cellular processes, mechanisms of action, and the effects of different treatments or interventions on cell health.