383372-69-8Relevant academic research and scientific papers
Fluorescence resonance energy transfer dye nucleotide terminators: A new synthetic approach for high-throughout DNA sequencing
Nampalli,Khot,Nelson,Flick,Fuller,Kumar
, p. 361 - 367 (2001)
Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10-13) were designed, synthesized, and formulated with Thermo Sequenase II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transfer (ET) rigid and linear linker (2), required for the syntheses of energy transfer cassettes (6-9) was synthesized via Heck coupling reaction on t-Boc-L-4-iodo-phenylalanine (1) with N-TFA-propargylamine.
