3878-83-9Relevant academic research and scientific papers
S-2-Hydroxyacylglutathione-Derivatives: Enzymatic Preparation, Purification and Characterisation
Clelland, James D.,Thornalley, Paul J.
, p. 3009 - 3016 (2007/10/02)
S-2-Hydroxyacylglutathione derivatives have been prepared by enzymatic synthesis from α-oxoaldehydes and reduced glutathione in the presence of glyoxalase I.S-D-Lactoylglutathione, S-D-mandelylglutathione, S-glycolylglutathione and S-L-glyceroylglutathione were prepared from methylglyoxal, phenylglyoxal, glyoxal and hydroxypyruvaldehyde, respectively.They were purified by ion exchange chromatography on Dowex 1 on a gram scale.Analytical data and re-evaluated extinction coefficients for these compounds are presented.The method described provides a reliable, large-scale procedure for the preparation and purification of S-acylglutathiones of increasing biological and pharmacological interest.
