388609-86-7Relevant academic research and scientific papers
Nucleotides (Part LXIX): Synthesis of phosphoramidite building blocks of isoxanthopterin n8-(2'-deoxy-β-dribonucleosides): New fluorescence markers for oligonucleotide synthesis
Lehbauer, Joerg,Pfleiderer, Wolfgang
, p. 2330 - 2342 (2007/10/03)
The chemical synthesis of isoxanthopterin and 6-phenylisoxanthopterin N8-(2'-deoxy-βD-ribofuranosyl nucleosides) is described as well as their conversion into suitably protected 3'-phosphoramidite building blocks to be used as marker molecules for DNA synthesis. Applying the npe/inpeoc (=2-(4-nitrophenyl)ethyl'[2-(4-nitrophenyl)ethoxy]carbonyl) strategy, we used the new building blocks in the preparation of oligonucleotides by an automated solid-support approach. The hybridization properties of a series of labelled oligomers were studied by UV-melting techniques. It was found that the newly synthesized markers only slightly interfered with the abilities of the labelled oligomers to form stable duplexes with complementary oligonucleotides.
