389625-49-4Relevant academic research and scientific papers
ZP4, an improved neuronal Zn2+ sensor of the Zinpyr family
Burdette, Shawn C.,Frederickson, Christopher J.,Bu, Weiming,Lippard, Stephen J.
, p. 1778 - 1787 (2007/10/03)
A second-generation fluorescent sensor for Zn2+ from the Zinpyr family, ZP4, has been synthesized and characterized, ZP4 (Zinpyr-4, 9-(o-carboxyphenyl)-2-chloro-5-[2-{bis(2-pyridylmethyl)-aminomethyl}- N-methylaniline]-6-hydroxy-3-xanthanone) is prepared via a convergent synthetic strategy developed from previous studies with these compounds, ZP4, like its predecessors, has excitation and emission wavelengths in the visible range (～500 nm), a dissociation constant (Kd) for Zn2+ of less than 1 nM and a high quantum yields (Π = ～0.4), making it well suited for biological applications, A 5-fold fluorescent enhancement is observed under simulated physiological conditions corresponding to the binding of the Zn2+ cation to the sensor, which inhibits a photoinduced electron transfer (PET) quenching pathway. The metal-binding stereochemistry of ZP4 was evaluated through the synthesis and X-ray structural characterization of [M(BPAMP)(H2O)n]+ complexes, where BPAMP is [2-{bis(2-pyridylmethyl)aminomethyl}-N-methylaniline]-phenol and M = Mn2+, Zn2+ (n = 1) or Cu2+ (n = 0).
