39445-21-1 Usage
Uses
Different sources of media describe the Uses of 39445-21-1 differently. You can refer to the following data:
1. Elastase is used in combination with other proteases for protein analysis by mass spectrometry. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. It is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.
2. Elastase from porcine pancreas has been used in a study to identify a novel bone/calcium metabolism-regulating factor in porcine pancreas. Elastase from porcine pancreas has also been used in a study to investigate the effect of a specific synthetic inhibitor of neutrophil elastase (ONO-5046) on the course of acute hemorrhagic pancreatitis in dogs.
3. Elastase from porcine pancreas has been used: to treat vero cells?to study its effects on syncytium formationas a positive control in protease assays as a component in RPMI 1640 to isolate human aortic smooth muscle cells (HASMCs) from the aortic tissue
General Description
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.
Biochem/physiol Actions
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.
Check Digit Verification of cas no
The CAS Registry Mumber 39445-21-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,9,4,4 and 5 respectively; the second part has 2 digits, 2 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 39445-21:
(7*3)+(6*9)+(5*4)+(4*4)+(3*5)+(2*2)+(1*1)=131
131 % 10 = 1
So 39445-21-1 is a valid CAS Registry Number.
