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39450-01-6 Usage


Proteinase K is an extracellular endopeptidase synthesized by the mold, Tritirachium album Limber. It belongs to a new subfamily of the subtilisins and is a 277 amino acid protein characterized by an unhydrolyzed protein chain and autolyzed polypeptide chains. It is a stable and highly reactive serine protease, known for its ability to degrade proteins and nucleic acids.


Used in Molecular Biology:
Proteinase K is used as a reagent for protein and nucleic acid isolation, particularly in the purification of RNA and DNA from tissues or cell lines. It aids in the proteolytic inactivation of nucleases during the isolation of DNA and RNA, ensuring the integrity of the extracted genetic material.
Used in Enzyme Localization:
Proteinase K is employed for the determination of enzyme localization on membranes, which is crucial for understanding the function and interaction of enzymes within cellular structures.
Used in Mitochondria Isolation:
It is utilized in the isolation of hepatic, yeast, and mung bean mitochondria, which are essential for studying cellular respiration and energy production.
Used in Endotoxin Removal:
Proteinase K is used to remove endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A, which can interfere with experimental results and pose potential health risks.
Used in Histology and Immunohistochemistry:
Proteinase K is involved in the treatment of paraffin-embedded tissue sections to expose antigen binding sites for antibody labeling, which is essential for histological analysis and immunohistochemical staining.
Used in Prion Research:
It is used for the digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research, helping to understand the mechanisms and progression of these neurodegenerative diseases.
Used in the Pharmaceutical Industry:
Proteinase K is used as a therapeutic agent for the treatment of various conditions, taking advantage of its proteolytic properties to target specific proteins involved in disease processes.
Used in the Food Industry:
In the food industry, Proteinase K is used as a processing aid for the tenderization of meat and the clarification of fruit juices, improving the texture and quality of the final products.
Used in the Cosmetic Industry:
Proteinase K is employed in the cosmetic industry for its exfoliating and skin-brightening properties, helping to improve skin texture and appearance.

Biochem/physiol Actions

Proteinase K catalyzes the hydrolysis of keratin.

Check Digit Verification of cas no

The CAS Registry Mumber 39450-01-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,9,4,5 and 0 respectively; the second part has 2 digits, 0 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 39450-01:
116 % 10 = 6
So 39450-01-6 is a valid CAS Registry Number.

39450-01-6 Well-known Company Product Price

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  • Sigma

  • (P6556)  Proteinase KfromTritirachiumalbum  lyophilized powder, ≥30 units/mg protein

  • 39450-01-6

  • P6556-5MG

  • 408.33CNY

  • Detail
  • Sigma

  • (P6556)  Proteinase KfromTritirachiumalbum  lyophilized powder, ≥30 units/mg protein

  • 39450-01-6

  • P6556-10MG

  • 465.66CNY

  • Detail
  • Sigma

  • (P6556)  Proteinase KfromTritirachiumalbum  lyophilized powder, ≥30 units/mg protein

  • 39450-01-6

  • P6556-25MG

  • 575.64CNY

  • Detail
  • Sigma

  • (P6556)  Proteinase KfromTritirachiumalbum  lyophilized powder, ≥30 units/mg protein

  • 39450-01-6

  • P6556-100MG

  • 1,208.61CNY

  • Detail
  • Sigma

  • (P6556)  Proteinase KfromTritirachiumalbum  lyophilized powder, ≥30 units/mg protein

  • 39450-01-6

  • P6556-500MG

  • 5,269.68CNY

  • Detail
  • Sigma

  • (P6556)  Proteinase KfromTritirachiumalbum  lyophilized powder, ≥30 units/mg protein

  • 39450-01-6

  • P6556-1G

  • 9,067.50CNY

  • Detail



According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 13, 2017

Revision Date: Aug 13, 2017


1.1 GHS Product identifier

Product name ProteinaseK from Tritirachium album

1.2 Other means of identification

Product number -
Other names -

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

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